This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-nCas9n
catalog :
47929
citations: 72
Reference
Yilmaz O, Com E, Pineau C, Bobe J. Genomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio). Sci Rep. 2023;13:18795 pubmed publisher
Moses E, Franěk R, Harel I. A scalable and tunable platform for functional interrogation of peptide hormones in fish. elife. 2023;12: pubmed publisher
Zhai Y, Zhang X, Zhao C, Geng R, Wu K, Yuan M, et al. Rescue of bmp15 deficiency in zebrafish by mutation of inha reveals mechanisms of BMP15 regulation of folliculogenesis. PLoS Genet. 2023;19:e1010954 pubmed publisher
Torraca V, Bielecka M, Gomes M, Brokatzky D, Busch Nentwich E, Mostowy S. Zebrafish null mutants of Sept6 and Sept15 are viable but more susceptible to Shigella infection. Cytoskeleton (Hoboken). 2023;: pubmed publisher
Chu W, Huang S, Chang C, Wu J, Gong H. Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing. Front Genet. 2023;14:1029200 pubmed publisher
Sieliwonczyk E, Vandendriessche B, Claes C, Mayeur E, Alaerts M, Holmgren P, et al. Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping. Sci Rep. 2023;13:1491 pubmed publisher
Khrystoforova I, Shochat Carvalho C, Harari R, Henke K, WORONOWICZ K, Harris M, et al. Zebrafish mutants reveal unexpected role of Lrp5 in osteoclast regulation. Front Endocrinol (Lausanne). 2022;13:985304 pubmed publisher
Oppel F, Shao S, Gendreizig S, Zimmerman M, Sch xfc rmann M, Flavian V, et al. p53 Pathway Inactivation Drives SMARCB1-deficient p53-wildtype Epithelioid Sarcoma Onset Indicating Therapeutic Vulnerability Through MDM2 Inhibition. Mol Cancer Ther. 2022;21:1689-1700 pubmed publisher
Zink M, Seewald A, Rohrbach M, Brodehl A, Liedtke D, Williams T, et al. Altered Expression of TMEM43 Causes Abnormal Cardiac Structure and Function in Zebrafish. Int J Mol Sci. 2022;23: pubmed publisher
Bearce E, Irons Z, Craig S, Kuhns C, Sabazali C, Farnsworth D, et al. Daw1 regulates the timely onset of cilia motility during development. Development. 2022;149: pubmed publisher
Wolińska Nizioł L, Romaniuk K, Wojciechowska K, Surga K, Kamaszewski M, Szudrowicz H, et al. Tollip-deficient zebrafish display no abnormalities in development, organ morphology or gene expression in response to lipopolysaccharide. FEBS Open Bio. 2022;: pubmed publisher
Hill E, Chen C, del Viso F, Ellington L, He S, Karabulut A, et al. Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis. Methods Mol Biol. 2022;2450:437-465 pubmed publisher
D Agostino Y, Frigato E, Noviello T, Toni M, Frabetti F, Cigliano L, et al. Loss of circadian rhythmicity in bdnf knockout zebrafish larvae. iScience. 2022;25:104054 pubmed publisher
Thumberger T, Tavhelidse Suck T, Gutierrez Triana J, Cornean A, Medert R, Welz B, et al. Boosting targeted genome editing using the hei-tag. elife. 2022;11: pubmed publisher
Ross K, Ren J, Zhang R, Chi N, Hamilton B. Ankfn1-mutant vestibular defects require loss of both ancestral and derived paralogs for penetrance in zebrafish. G3 (Bethesda). 2022;12: pubmed publisher
Song Y, Chen W, Zhu B, Ge W. Disruption of Epidermal Growth Factor Receptor but Not EGF Blocks Follicle Activation in Zebrafish Ovary. Front Cell Dev Biol. 2021;9:750888 pubmed publisher
Mohsin M, Li Y, Zhang X, Wang Y, Huang Z, Yin G, et al. Development of CRISPR-CAS9 based RNA drugs against Eimeria tenella infection. Genomics. 2021;113:4126-4135 pubmed publisher
Ogawa Y, Shiraki T, Fukada Y, Kojima D. Foxq2 determines blue cone identity in zebrafish. Sci Adv. 2021;7:eabi9784 pubmed publisher
Jang H, Chen Y, Ge J, Wilkening A, Hou Y, Lee H, et al. Epigenetic dynamics shaping melanophore and iridophore cell fate in zebrafish. Genome Biol. 2021;22:282 pubmed publisher
Madden M, Suminaite D, Ortiz E, Early J, Koudelka S, Livesey M, et al. CNS Hypomyelination Disrupts Axonal Conduction and Behavior in Larval Zebrafish. J Neurosci. 2021;41:9099-9111 pubmed publisher
Ferreira F, Carvalho L, Logarinho E, Bessa J. foxm1 Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis. Cells. 2021;10: pubmed publisher
Hamar J, Kültz D. An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters. Sci Rep. 2021;11:7854 pubmed publisher
Mero S, Salviati L, Leuzzi V, Rubegni A, Calderan C, Nardecchia F, et al. New pathogenic variants in COQ4 cause ataxia and neurodevelopmental disorder without detectable CoQ10 deficiency in muscle or skin fibroblasts. J Neurol. 2021;268:3381-3389 pubmed publisher
Arias Padilla L, Castañeda Cortés D, Rosa I, Moreno Acosta O, Hattori R, Nóbrega R, et al. Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka. elife. 2021;10: pubmed publisher
Amorim J, Bordeira Carriço R, Gali Macedo A, Perrod C, Bessa J. CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish. STAR Protoc. 2020;1:100208 pubmed publisher
Zinani O, Keseroglu K, Ay A, Ozbudak E. Pairing of segmentation clock genes drives robust pattern formation. Nature. 2021;589:431-436 pubmed publisher
Shochat C, Wang Z, Mo C, Nelson S, Donaka R, Huang J, et al. Deletion of SREBF1, a Functional Bone-Muscle Pleiotropic Gene, Alters Bone Density and Lipid Signaling in Zebrafish. Endocrinology. 2021;162: pubmed publisher
Oppel F, Ki D, Zimmermann M, Ross K, Tao T, Shi H, et al. suz12 inactivation in p53 and nf1 deficient zebrafish accelerates the onset of MPNSTs and expands the spectrum of tumor types to include adenocarcinoma, leukemia, and soft tissue sarcoma. Dis Model Mech. 2020;: pubmed publisher
Bottiglione F, Dee C, Lea R, Zeef L, Badrock A, Wane M, et al. Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis. J Immunol. 2020;: pubmed publisher
Pan Y, Sun Y, Wang Y, Zhang Z. Barcode sequence could be a good target for developing a species-specific anti-parasite agent based on CRISPR-Cas9. FASEB J. 2020;: pubmed publisher
Badrock A, Uggenti C, Wacheul L, Crilly S, Jenkinson E, Rice G, et al. Analysis of U8 snoRNA Variants in Zebrafish Reveals How Bi-allelic Variants Cause Leukoencephalopathy with Calcifications and Cysts. Am J Hum Genet. 2020;106:694-706 pubmed publisher
Meneghetti G, Skobo T, Chrisam M, Fontana C, Facchinello N, Nazio F, et al. Zebrafish ambra1a and ambra1b Silencing Affect Heart Development. Zebrafish. 2020;: pubmed publisher
Ye C, Chen Z, Liu Z, Wang F, He X. Defining endogenous barcoding sites for CRISPR/Cas9-based cell lineage tracing in zebrafish. J Genet Genomics. 2020;47:85-91 pubmed publisher
Weuring W, Singh S, Volkers L, Rook M, van t Slot R, Bosma M, et al. NaV1.1 and NaV1.6 selective compounds reduce the behavior phenotype and epileptiform activity in a novel zebrafish model for Dravet Syndrome. PLoS ONE. 2020;15:e0219106 pubmed publisher
Idigo N, Soares D, Abbott C. Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish. Biosci Rep. 2020;40: pubmed publisher
Oprişoreanu A, Smith H, Arya S, Webster R, Zhong Z, Wehner D, et al. Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Cell Rep. 2019;29:1082-1098.e10 pubmed publisher
Liedtke D, Orth M, Meissler M, Geuer S, Knaup S, Köblitz I, et al. ECM alterations in Fndc3a (Fibronectin Domain Containing Protein 3A) deficient zebrafish cause temporal fin development and regeneration defects. Sci Rep. 2019;9:13383 pubmed publisher
Xie Y, Gao L, Xu C, Chu L, Gao L, Wu R, et al. ARHGEF12 regulates erythropoiesis and is involved in erythroid regeneration after chemotherapy in acute lymphoblastic leukemia patients. Haematologica. 2020;105:925-936 pubmed publisher
Bose P, Tremblay E, Maois C, Narasimhan V, Armstrong G, Liao M, et al. The Novel Small Molecule TRVA242 Stabilizes Neuromuscular Junction Defects in Multiple Animal Models of Amyotrophic Lateral Sclerosis. Neurotherapeutics. 2019;: pubmed publisher
Vázquez Marín J, Gutierrez Triana J, Almuedo Castillo M, Buono L, Gomez Skarmeta J, Mateo J, et al. yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets. Development. 2019;146: pubmed publisher
Grainger S, Nguyen N, Richter J, Setayesh J, Lonquich B, Oon C, et al. EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells. Nat Cell Biol. 2019;: pubmed publisher
Abe M, Kuroda R. The development of CRISPR for a mollusc establishes the formin Lsdia1 as the long-sought gene for snail dextral/sinistral coiling. Development. 2019;146: pubmed publisher
Princely Abudu Y, Pankiv S, Mathai B, Håkon Lystad A, Bindesbøll C, Brenne H, et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell. 2019;49:509-525.e12 pubmed publisher
Tessadori F, Roessler H, Savelberg S, Chocron S, Kamel S, Duran K, et al. Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders. Dis Model Mech. 2018;11: pubmed publisher
Julien D, Chan A, BARRIOS J, Mathiaparanam J, Douglass A, Wolman M, et al. Zebrafish expression reporters and mutants reveal that the IgSF cell adhesion molecule Dscamb is required for feeding and survival. J Neurogenet. 2018;32:336-352 pubmed publisher
Gay S, Bugeon J, Bouchareb A, Henry L, Delahaye C, Legeai F, et al. MiR-202 controls female fecundity by regulating medaka oogenesis. PLoS Genet. 2018;14:e1007593 pubmed publisher
Casar B, Badrock A, Jiménez I, Arozarena I, Colón Bolea P, Lorenzo Martín L, et al. RAS at the Golgi antagonizes malignant transformation through PTPRκ-mediated inhibition of ERK activation. Nat Commun. 2018;9:3595 pubmed publisher
Guner Ataman B, González Rosa J, Shah H, Butty V, Jeffrey S, Abrial M, et al. Failed Progenitor Specification Underlies the Cardiopharyngeal Phenotypes in a Zebrafish Model of 22q11.2 Deletion Syndrome. Cell Rep. 2018;24:1342-1354.e5 pubmed publisher
de Vos I, Tao E, Ong S, Goggi J, Scerri T, Wilson G, et al. Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype. Hum Mol Genet. 2018;27:2775-2788 pubmed publisher
Chen J, Wang W, Tian Z, Dong Y, Dong T, Zhu H, et al. Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus). Front Genet. 2018;9:117 pubmed publisher
Alemany A, Florescu M, Baron C, Peterson Maduro J, van Oudenaarden A. Whole-organism clone tracing using single-cell sequencing. Nature. 2018;556:108-112 pubmed publisher
Hall C, Sanderson L, Lawrence L, Pool B, van der Kroef M, Ashimbayeva E, et al. Blocking fatty acid-fueled mROS production within macrophages alleviates acute gouty inflammation. J Clin Invest. 2018;128:1752-1771 pubmed publisher
Kell M, Riccio R, Baumgartner E, Compton Z, Pecorin P, Mitchell T, et al. Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1). PLoS ONE. 2018;13:e0190353 pubmed publisher
Guo W, Xie B, Xiong S, Liang X, Gui J, Mei J. miR-34a Regulates Sperm Motility in Zebrafish. Int J Mol Sci. 2017;18: pubmed publisher
Pelliccia J, Jindal G, Burdine R. Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning. elife. 2017;6: pubmed publisher
Han M, Van Der Krogt G, de Rooij J. Zygotic vinculin is not essential for embryonic development in zebrafish. PLoS ONE. 2017;12:e0182278 pubmed publisher
Facchinello N, Skobo T, Meneghetti G, Colletti E, Dinarello A, Tiso N, et al. nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Sci Rep. 2017;7:4371 pubmed publisher
He X, Zhang W, Yan C, Nie F, Li C, Liu X, et al. Chemical biology reveals CARF as a positive regulator of canonical Wnt signaling by promoting TCF/β-catenin transcriptional activity. Cell Discov. 2017;3:17003 pubmed publisher
Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C, et al. ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Nat Commun. 2017;8:14714 pubmed publisher
Morris E, Bodin S, Delaval B, Comunale F, Georget V, Costa M, et al. Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion. Biol Cell. 2017;109:210-221 pubmed publisher
Antosova B, Smolikova J, Klimova L, Lachova J, Bendova M, Kozmikova I, et al. The Gene Regulatory Network of Lens Induction Is Wired through Meis-Dependent Shadow Enhancers of Pax6. PLoS Genet. 2016;12:e1006441 pubmed publisher
Lau E, Zhang Z, Qin M, Ge W. Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation. Sci Rep. 2016;6:37357 pubmed publisher
Harel I, Valenzano D, Brunet A. Efficient genome engineering approaches for the short-lived African turquoise killifish. Nat Protoc. 2016;11:2010-2028 pubmed publisher
Yoo S, Pascoe H, Pereira T, Kondo S, Jacinto A, Zhang X, et al. Plexins function in epithelial repair in both Drosophila and zebrafish. Nat Commun. 2016;7:12282 pubmed publisher
Dehler C, Boudinot P, Martin S, Collet B. Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line. Mar Biotechnol (NY). 2016;18:449-52 pubmed publisher
Armstrong G, Liao M, You Z, Lissouba A, Chen B, Drapeau P. Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System. PLoS ONE. 2016;11:e0150188 pubmed publisher
Ogawa Y, Shiraki T, Kojima D, Fukada Y. Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Proc Biol Sci. 2015;282:20150659 pubmed publisher
Gao L, Li D, Ma K, Zhang W, Xu T, Fu C, et al. TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. PLoS Genet. 2015;11:e1005346 pubmed publisher
Willems B, Tao S, Yu T, Huysseune A, Witten P, Winkler C. The Wnt Co-Receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish. PLoS ONE. 2015;10:e0131768 pubmed publisher
Harel I, Benayoun B, Machado B, Singh P, Hu C, Pech M, et al. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate. Cell. 2015;160:1013-1026 pubmed publisher
Shin J, Chen J, Solnica Krezel L. Efficient homologous recombination-mediated genome engineering in zebrafish using TALE nucleases. Development. 2014;141:3807-18 pubmed publisher
Jao L, Wente S, Chen W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013;110:13904-9 pubmed publisher
product information
Catalog Number :
47929
Product Name :
pCS2-nCas9n
article :
doi10.1073/pnas.1308335110
id7188
pubmed_id23918387
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
Note from depositor: Although this plasmid produces smaller transcripts in addition to the expected one, the product mixture displays activity comparable to that from pT3TS-nCas9n. For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
growth strain :
expression of an optimized Cas9 for genome-editing in zebrafish
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3M13 Rev
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namenls-zcas9-nls
shRNA_sequence
size4200
species
100
Synthetic
tags
resistance markers :
1611
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA