This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
catalog :
47868
citations: 6
Reference
Moon B, Huang D, Gao F, Cai M, Lyu G, Zhang L, et al. Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency. Cell Death Discov. 2023;9:61 pubmed publisher
Song G, Zhang F, Zhang X, Gao X, Zhu X, Fan D, et al. AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage. Cell Rep. 2019;29:2579-2589.e4 pubmed publisher
Amrani N, Gao X, Liu P, Edraki A, Mir A, Ibraheim R, et al. NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol. 2018;19:214 pubmed publisher
Yang D, Scavuzzo M, Chmielowiec J, Sharp R, Bajić A, Borowiak M. Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Sci Rep. 2016;6:21264 pubmed publisher
Lee C, Cradick T, Bao G. The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells. Mol Ther. 2016;24:645-54 pubmed publisher
Hou Z, Zhang Y, Propson N, Howden S, Chu L, Sontheimer E, et al. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013;110:15644-9 pubmed publisher
product information
Catalog Number :
47868
Product Name :
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
article :
doi10.1073/pnas.1313587110
id7254
pubmed_id23940360
bacterial resistance :
Ampicillin
cloning :
backbonepSimpleII
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
origin :
37
pi :
alt_names
CSN1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameNmCas9
shRNA_sequence
size3243
species
32644
Other
N meningitidis
tags
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
locationC terminal on insert
tagHA
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterU6
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameU6pr-tracrRNA
shRNA_sequence
size364
species
32644
Other
N meningitidis
tags
resistance markers :
1713
523
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA