This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSimpleII-NLS-NmCas9-HA-NLS(s)
catalog :
47867
citations: 1
Reference
Hou Z, Zhang Y, Propson N, Howden S, Chu L, Sontheimer E, et al. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013;110:15644-9 pubmed publisher
product information
Catalog Number :
47867
Product Name :
pSimpleII-NLS-NmCas9-HA-NLS(s)
article :
doi10.1073/pnas.1313587110
id7254
pubmed_id23940360
bacterial resistance :
Ampicillin
cloning :
backbonepSimpleII
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
This plasmid encode NmCas9 with two NLS and a HA tag
origin :
37
pi :
alt_names
CSN1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameNmCas9
shRNA_sequence
size3243
species
32644
Other
N meningitidis
tags
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
locationC terminal on insert
tagHA
resistance markers :
1713
523
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA