This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNCMV 18E6no* I130T
catalog :
47866
citations: 1
Reference
Spangle J, Ghosh Choudhury N, Munger K. Activation of cap-dependent translation by mucosal human papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif. J Virol. 2012;86:7466-72 pubmed publisher
product information
Catalog Number :
47866
Product Name :
pNCMV 18E6no* I130T
article :
doi10.1128/JVI.00487-12
id5488
pubmed_id22553330
bacterial resistance :
Ampicillin
cloning :
backbonepNCMV
backbone_mutation
backbone_origin
backbone_size6550
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
**The V44A mutation prevents the internal splicing of the DNA so only the no * (unspliced form) of 18E6 is produced.
growth strain :
Defective for E6AP binding
origin :
37
pi :
alt_names
HPV 18E6
18 E6
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3CMV bam neo 3'
sequencing_primer_5CMV bam neo 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
NC_001357.1
mutationI130T, V44A*
nameHPV 18 E6no*
shRNA_sequence
size471
species
32644
Other
HPV
tags
locationN terminal on backbone
tagFLAG
locationN terminal on backbone
tagHA
resistance markers :
351
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA