This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSP1-bio
catalog :
47709
citations: 2
Reference
Gonzales S, Clarke K, Batugedara G, Garza R, Braddom A, Reyes R, et al. A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From Uganda. Front Immunol. 2022;13:809264 pubmed publisher
Crosnier C, Wanaguru M, McDade B, Osier F, Marsh K, Rayner J, et al. A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins. Mol Cell Proteomics. 2013;12:3976-86 pubmed publisher
product information
Catalog Number :
47709
Product Name :
MSP1-bio
article :
doi10.1074/mcp.O113.028357
id7418
pubmed_id24043421
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutation
backbone_originYves Durocher, NRC
backbone_size6633
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPFI1475w, MSA1, MSP-1, PMMSA, Pf190, merozoite surface antigen 1
geneMSP1
id813575
genbank_ids
mutationExogenous signal peptide of mouse origin, changed Serine123, Threonine262, Threonine491, Serine557, Serine628, Serine823, Threonine835, Threonine920, Serine940, Threonine986, Threonine1012, Serine1110, Threonine1217, Serine1609 to Alanine residues
nameCodon-optimised MSP1
shRNA_sequence
size5046
species
100
Synthetic
32644
Other
Plasmodium falciparum
tags
locationC terminal on backbone
tagrat Cd4 d3+4
locationC terminal on backbone
tagenzymatic biotinylation sequence
plasmid copy :
pTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
resistance markers :
919
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA