This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmalc4x_K398R_TEV
catalog :
47701
citations: 1
Reference
Burns J, Turnage K, Walker C, Lieberman R. The stability of myocilin olfactomedin domain variants provides new insight into glaucoma as a protein misfolding disorder. Biochemistry. 2011;50:5824-33 pubmed publisher
product information
Catalog Number :
47701
Product Name :
pmalc4x_K398R_TEV
article :
doi10.1021/bi200231x
id7134
pubmed_id21612213
bacterial resistance :
Ampicillin
cloning :
backbonepmalC4X
backbone_mutation
backbone_originNEB
backbone_size6645
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
growth strain :
Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
origin :
37
pi :
alt_names
K398R
OLF
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5Unknown
promoterPtac
sequencing_primer_3M13-F20; M13_pUC_Fwd
sequencing_primer_5MBP-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGLC1A, GPOA, JOAG, JOAG1, TIGR
geneMYOC
id4653
genbank_ids
mutationchanged Lysine 398 to Arginine
nameOlfactomedin-like domain with K398R mutation
shRNA_sequence
size837
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMBP
resistance markers :
1676
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA