This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
nes714 1388-1507tk/lacZ
catalog :
47616
citations: 1
Reference
Lothian C, Prakash N, Lendahl U, Wahlström G. Identification of both general and region-specific embryonic CNS enhancer elements in the nestin promoter. Exp Cell Res. 1999;248:509-19 pubmed
product information
Catalog Number :
47616
Product Name :
nes714 1388-1507tk/lacZ
article :
doi10.1006/excr.1999.4417
id7119
pubmed_id10222142
bacterial resistance :
Ampicillin
cloning :
backboneSaStk/lacZ
backbone_mutationremoved SalI site from pTKbeta and replaced HSV tk promoter with a basic HSV tk promoter containing SalI site.
backbone_originClontech; modified by Lendahl lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
enhancer reporter
growth notes :
HindIII cleavage excises the complete enhancer-tk promoter-lacZ fragment from the vector. The lacZ gene can be removed from the SaStk/lacZ vector after cleavage with NotI.
growth strain :
used to create transgenic mice expressing LacZ reporter with Nestin enhancer
origin :
37
pi :
alt_names
NES
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SalI
promoterHSV tk promoter
sequencing_primer_3LacZ-R
sequencing_primer_5SaStk/lacZ 5 (gtcggggctggcttaactat)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNbla00170
geneNES
id10763
genbank_ids
mutation714 bp from 3' end of 2nd intron with a deletion of nt 1388-1507
namenestin 2nd intron fragment
shRNA_sequence
size595
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHSV tk promoter
locationC terminal on backbone
taglacZ
resistance markers :
1677
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA