nes714tk/lacZ | Addgene 47614 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

nes714tk/lacZ

catalog :

47614

citations: 2

Reference
Middelkamp S, van Heesch S, Braat A, de Ligt J, van Iterson M, Simonis M, et al. Molecular dissection of germline chromothripsis in a developmental context using patient-derived iPS cells. Genome Med. 2017;9:9 pubmed publisher
Lothian C, Lendahl U. An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells. Eur J Neurosci. 1997;9:452-62 pubmed

product information

Catalog Number :

47614

Product Name :

nes714tk/lacZ

article :

doi	10.1111/j.1460-9568.1997.tb01622.x
id	7118
pubmed_id	9104587

bacterial resistance :

Ampicillin

cloning :

backbone

SaStk/lacZ

backbone_mutation

removed SalI site from pTKbeta and replaced HSV tk promoter with a basic HSV tk promoter containing SalI site.

backbone_origin

Clontech; modified by Lendahl lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

enhancer reporter

growth notes :

HindIII cleavage excises the complete enhancer-tk promoter-lacZ fragment from the vector. The lacZ gene can be removed from the SaStk/lacZ vector after cleavage with NotI.

growth strain :

used to create transgenic mice expressing LacZ reporter with Nestin enhancer

origin :

pi :

alt_names

NES

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	SalI
promoter	HSV tk promoter
sequencing_primer_3	LacZ-R
sequencing_primer_5	SaStk/lacZ 5 (gtcggggctggcttaactat)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Nbla00170
gene	NES
id	10763

genbank_ids

mutation

714 bp from 3' end of 2nd intron

name

nestin 2nd intron fragment

shRNA_sequence

size

714

species

9606

Homo sapiens