This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T-1 3xHA-MKK6-EE
catalog :
47579
citations: 1
Reference
Bose A, Janes K. A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Mol Cell Proteomics. 2013;12:797-806 pubmed publisher
product information
Catalog Number :
47579
Product Name :
pGEX-4T-1 3xHA-MKK6-EE
article :
doi10.1074/mcp.O112.024059
id7106
pubmed_id23233447
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T-1
backbone_mutation3xHA tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
backbone_originAmersham
backbone_size5062
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6
inserts :
BL21
origin :
37
pi :
alt_names
MAP2K6
MEK6
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pGEX 3'
sequencing_primer_5pGEX 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesMAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3
geneMAP2K6
id5608
genbank_ids
NM_002758
mutationSer207 to Glu, Thr211 to Glu
nameMKK6-EE
shRNA_sequence
size1005
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
locationN terminal on backbone
tag3xHA
plasmid copy :
Addgene plasmid #13518.
resistance markers :
1585
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA