This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T-1 3xFlag-JNK1a1
catalog :
47574
citations: 2
Reference
Solone X, Caldara A, Wells B, Qiao H, Wade L, Salerno J, et al. MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. FEBS Open Bio. 2022;12:1075-1086 pubmed publisher
Bose A, Janes K. A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Mol Cell Proteomics. 2013;12:797-806 pubmed publisher
product information
Catalog Number :
47574
Product Name :
pGEX-4T-1 3xFlag-JNK1a1
article :
doi10.1074/mcp.O112.024059
id7106
pubmed_id23233447
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T-1
backbone_mutation3xFlag tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
backbone_originAmersham
backbone_size5053
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1
inserts :
BL21
origin :
37
pi :
alt_names
MAPK8
SAPK1
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pGEX 3'
sequencing_primer_5pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesJNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
geneMAPK8
id5599
genbank_ids
NM_002750
mutation
nameJNK1a1
shRNA_sequence
size1155
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
locationN terminal on backbone
tag3xFlag
plasmid copy :
Addgene plasmid #13798.
resistance markers :
1585
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA