This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T-1 3xFlag-ERK2
catalog :
47573
citations: 3
Reference
Lai K, Galan S, Zeng Y, Zhou T, He C, Raj R, et al. LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome. Cell. 2021;184:2680-2695.e26 pubmed publisher
Courtney T, Deiters A. Optical control of protein phosphatase function. Nat Commun. 2019;10:4384 pubmed publisher
Bose A, Janes K. A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Mol Cell Proteomics. 2013;12:797-806 pubmed publisher
product information
Catalog Number :
47573
Product Name :
pGEX-4T-1 3xFlag-ERK2
article :
doi10.1074/mcp.O112.024059
id7106
pubmed_id23233447
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T-1
backbone_mutation3xFlag tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
backbone_originAmersham
backbone_size5053
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression plasmid for GST-3xFlag-tagged ERK2
inserts :
BL21
origin :
37
pi :
alt_names
MAPK1
p42-MAPK
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pGEX 3'
sequencing_primer_5pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERK-2, ERT1, Erk2, p42-MAPK
geneMapk1
id116590
genbank_ids
NM_053842
mutation
nameERK2
shRNA_sequence
size1077
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGST
locationN terminal on backbone
tag3xFlag
plasmid copy :
Addgene plasmid #8974.
resistance markers :
1585
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA