This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-KIF17-short-pcDNA3
catalog :
47545
product information
Catalog Number :
47545
Product Name :
FLAG-KIF17-short-pcDNA3
article :
| doi | |
| id | 7101 |
| pubmed_id |
bacterial resistance :
Ampicillin
cloning :
| backbone | pcDNA3 | |
| backbone_mutation | ||
| backbone_origin | Invitrogen | |
| backbone_size | 5546 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
This vector directs expression of neither of the current mouse RefSeq Kif17 variants, isoform 1 or isoform 2. Rather the vector expresses a cDNA which corresponds to a different alternatively spliced transcript encoding a still shorter isoform of 511 amino acids rather than the 1038 residues of isoform 1 or 710 of isoform 2. The first 400 residues of the short isoform are essentially identical to the amino-terminal motor domain of isoform 1 and 2. Based on studies of Kif17 truncations, the short form of mouse Kif17 is predicted to form dimers and to bind microtubules. *Compared to the reference sequence, this expression vector expresses a protein with substitution of conserved glycine-152 with a serine in the motor domain of Kif17. Due to this mutation, the properties of the produced protein could be altered.
growth strain :
Expresses FLAG epitope-tagged short form of mouse KIF17 in mammalian cells
origin :
37
pi :
|
resistance markers :
| 1631 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments