pVC299 VEGF Site#2 | Addgene 47506 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pVC299 VEGF Site#2

catalog :

47506

citations: 3

Reference
Rose J, Popp N, Richardson C, Stephany J, Mathieu J, Wei C, et al. Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs. Nat Commun. 2020;11:2697 pubmed publisher
Rose J, Stephany J, Valente W, Trevillian B, Dang H, Bielas J, et al. Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics. Nat Methods. 2017;14:891-896 pubmed publisher
Fu Y, Foden J, Khayter C, Maeder M, Reyon D, Joung J, et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013;31:822-6 pubmed publisher

product information

Catalog Number :

47506

Product Name :

pVC299 VEGF Site#2

article :

doi	10.1038/nbt.2623
id	7091
pubmed_id	23792628

bacterial resistance :

Ampicillin

cloning :

backbone

MLM3636

backbone_mutation

backbone_origin

Joung lab (Addgene plasmid #43860)

backbone_size

2278

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

Target site: GACCCCCTCCACCCCGCCTC gRNA expression vector targeting human VEGF for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9) For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

growth strain :

human gRNA expression vector targeting VEGF

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BsmBI
cloning_site_5	BsmBI
promoter	U6
sequencing_primer_3
sequencing_primer_5	hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	L-VEGF, MVCD1, VEGF, VPF
gene	VEGFA
id	7422

genbank_ids

mutation

name

gRNA-VEGF site 2

shRNA_sequence

size

species

9606

Homo sapiens