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product summary
company name :
Addgene
product type :
cDNA
product name :
MIGR1-cyclin E-AA
catalog :
47498
citations: 1
product information
Catalog Number :
47498
Product Name :
MIGR1-cyclin E-AA
article :
| doi | 10.1038/onc.2013.289 |
| id | 7086 |
| pubmed_id | 23873023 |
bacterial resistance :
Ampicillin
cloning :
| backbone | MIGR1 | ||||
| backbone_mutation | none | ||||
| backbone_origin | |||||
| backbone_size | 6521 | ||||
| promoter | |||||
| sequencing_primer_3 | |||||
| sequencing_primer_5 | |||||
| vector_types |
|
growth notes :
Human cyclin E1 (CCNE1) cDNA was mutated at N- and C-terminal Cdc4 phosphodegrons (T62A and T380A, see references) to disrupt interaction with Fbw7 ubiquitin ligase. cDNA is inserted into MIGR1 (MSCV-based retroviral vector), permitting transduction of hematopoietic cells. Cloned cDNA contains approximately 100 bp from CCNE1 3' UTR. GFP is co-expressed with cDNA via IRES, permitting enrichment for transduced cells using flow sorting. References: Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW et al (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294: 173-177. Strohmaier H, Spruck CH, Kaiser P, Won KA, Sangfelt O, Reed SI (2001). Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line. Nature 413: 316-322.
growth strain :
MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons
origin :
37
pi :
|
resistance markers :
| 821 |
tags :
High Copy
terms :
| Other |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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