pCDNA_B2AR-s-pep | Addgene 47438 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA_B2AR-s-pep

catalog :

47438

citations: 3

Reference
Semack A, Malik R, Sivaramakrishnan S. G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay. J Vis Exp. 2016;: pubmed publisher
Khan M, Lustrino D, Silveira W, Wild F, Straka T, Issop Y, et al. Sympathetic innervation controls homeostasis of neuromuscular junctions in health and disease. Proc Natl Acad Sci U S A. 2016;113:746-50 pubmed publisher
Malik R, Ritt M, DeVree B, Neubig R, Sunahara R, Sivaramakrishnan S. Detection of G protein-selective G protein-coupled receptor (GPCR) conformations in live cells. J Biol Chem. 2013;288:17167-78 pubmed publisher

product information

Catalog Number :

47438

Product Name :

pCDNA_B2AR-s-pep

article :

doi	10.1074/jbc.M113.464065
id	7079
pubmed_id	23629648

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA5_FRT

backbone_mutation

backbone_origin

backbone_size

5070

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Notes: (1) The pCDNA/FRT vector contains an extra XbaI site. (2) The XbaI restriction enzyme is sensitive to dam methylation.

growth strain :

FRET sensor for beta2-adrenergic receptor

origin :

pi :

alt_names

ADRB2

beta2-AR

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	HindIII
promoter	pCMV
sequencing_primer_3	BGHR Reverse
sequencing_primer_5	T7 Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
gene	ADRB2
id	154

genbank_ids

NM_000024.5

mutation

name

beta2-adrenergic receptor

shRNA_sequence

size

3181

species

9606

Homo sapiens