pCDNA_B2AR-i-pep | Addgene 47436 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA_B2AR-i-pep

catalog :

47436

citations: 2

Reference
Vidigal J, Fernandes B, Dias M, Patrone M, Roldão A, Carrondo M, et al. RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles. Appl Microbiol Biotechnol. 2018;102:655-666 pubmed publisher
Malik R, Ritt M, DeVree B, Neubig R, Sunahara R, Sivaramakrishnan S. Detection of G protein-selective G protein-coupled receptor (GPCR) conformations in live cells. J Biol Chem. 2013;288:17167-78 pubmed publisher

product information

Catalog Number :

47436

Product Name :

pCDNA_B2AR-i-pep

article :

doi	10.1074/jbc.M113.464065
id	7079
pubmed_id	23629648

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA5_FRT

backbone_mutation

backbone_origin

backbone_size

5070

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Notes: (1) The pCDNA/FRT vector contains an extra XbaI site. (2) The XbaI restriction enzyme is sensitive to dam methylation.

growth strain :

FRET sensor for beta2-adrenergic receptor

origin :

pi :

alt_names

ADRB2

beta2-AR

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	HindIII
promoter	pCMV
sequencing_primer_3	BGHR Reverse
sequencing_primer_5	T7 Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
gene	ADRB2
id	154

genbank_ids

NM_000024.5

mutation

name

beta2-adrenergic receptor

shRNA_sequence

size

3181

species

9606

Homo sapiens