This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA5-EGFP-AID-BubR1
catalog :
47330
citations: 5
Reference
Aksenova V, Arnaoutov A, Dasso M. Analysis of Nucleoporin Function Using Inducible Degron Techniques. Methods Mol Biol. 2022;2502:129-150 pubmed publisher
Brooks K, Daughtry B, Davis B, Yan M, Fei S, Shepherd S, et al. Molecular contribution to embryonic aneuploidy and karyotypic complexity in initial cleavage divisions of mammalian development. Development. 2022;149: pubmed publisher
Aksenova V, Smith A, Lee H, Bhat P, Esnault C, Chen S, et al. Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway. Nat Commun. 2020;11:4577 pubmed publisher
Li X, Li L, Li J, Sipple J, Schick J, Mehta P, et al. Concomitant inactivation of foxo3a and fancc or fancd2 reveals a two-tier protection from oxidative stress-induced hydrocephalus. Antioxid Redox Signal. 2014;21:1675-92 pubmed publisher
Holland A, Fachinetti D, Han J, Cleveland D. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012;109:E3350-7 pubmed publisher
product information
Catalog Number :
47330
Product Name :
pcDNA5-EGFP-AID-BubR1
article :
doi10.1073/pnas.1216880109
id7057
pubmed_id23150568
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5/FRT/TO
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Nishimura et al., 2009, Nature Methods
growth strain :
for plasmid integration using the Flp-In system. AID at N-terminal
origin :
37
pi :
alt_names
Bub1B
BubR1
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5BamHI
promoter
sequencing_primer_3ACT CGA GCG GCC GCC TAT CAC TGA AAG AGC AAA GCC CCA GGA CTA GTT AA
sequencing_primer_5ACT AGT GGA TCC CTA TCA CTG AAA GAG CAA AGC CCC AGG ACT AGT TAA CT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1
geneBUB1B
id701
genbank_ids
NM_001211.5
mutationsiRNA resistant
nameBUB1 mitotic checkpoint serine/threonine kinase B
shRNA_sequence
size4590
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
locationN terminal on insert
tagAID
plasmid copy :
The AID ORF was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
resistance markers :
1665
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA