pcDNA5-H2B-AID-EYFP | Addgene 47329 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA5-H2B-AID-EYFP

catalog :

47329

citations: 3

Reference
Noviello G, Gjaltema R, Schulz E. CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Nat Commun. 2023;14:3225 pubmed publisher
Kubo N, Ishii H, Xiong X, Bianco S, Meitinger F, Hu R, et al. Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation. Nat Struct Mol Biol. 2021;28:152-161 pubmed publisher
Holland A, Fachinetti D, Han J, Cleveland D. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012;109:E3350-7 pubmed publisher

product information

Catalog Number :

47329

Product Name :

pcDNA5-H2B-AID-EYFP

article :

doi	10.1073/pnas.1216880109
id	7057
pubmed_id	23150568

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Nishimura et al., 2009, Nature Methods

growth strain :

for plasmid integration using the Flp-In system. AID at C-terminal

origin :

pi :

H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BEgeneH2BC21id8349genbank_ids

NP_066402.2

mutationnamehistone H2BshRNA_sequencesize6875species

9606

Homo sapiens

tags

location	C terminal on insert
tag	AID

location	C terminal on insert
tag	EYFP

GGsequencing_primer_5
CTTAAGCTTACCGGTGCCGCCACCatgcca
gagccagcgaagtctgctccs
ite_3_destroyed<
td>site_5_destroyed<
/table>entrez_gened>

ali
ases

GL105,

alt_names

H2B

cloning

clone_method

Restriction Enzyme

cloning_site_3

XmaI

cloning_site_5

HindIII

promoter

sequencing_primer_3

CAT CCC GGG CTT AGC GCT GGT GTA CTT GGT GAT GGC CTT AGT ACC CTC

plasmid copy :

The AID ORF was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).

resistance markers :

1665

tags :

High Copy

terms :

Hygromycin