This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-28b-Cas9-His
catalog :
47327
citations: 26
Reference
Zhang W, Petri K, Ma J, Lee H, Tsai C, Joung J, et al. Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions. bioRxiv. 2023;: pubmed publisher
Zadabbas Shahabadi H, Akbarzadeh A, Ofoghi H, Kadkhodaei S. Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR. Front Plant Sci. 2023;14:1150436 pubmed publisher
Baidukova O, Oppermann J, Kelterborn S, Fernandez Lahore R, Schumacher D, Evers H, et al. Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation. Nat Commun. 2022;13:7253 pubmed publisher
Yokomori M, Suzuki H, Nakamura A, Sugano S, Tagawa M. DNA-functionalized colloidal crystals for macromolecular encapsulation. Soft Matter. 2022;18:6954-6964 pubmed publisher
Lainšček D, Forstnerič V, Mikolič V, Malenšek Š, Pečan P, Bencina M, et al. Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing. Nat Commun. 2022;13:3604 pubmed publisher
Letelier J, Naranjo S, Sospedra Arrufat I, Martinez Morales J, Lopez Rios J, Shubin N, et al. The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Zhang Y, Iaffaldano B, Qi Y. CRISPR ribonucleoprotein-mediated genetic engineering in plants. Plant Commun. 2021;2:100168 pubmed publisher
Ghetti S, Burigotto M, Mattivi A, Magnani G, Casini A, Bianchi A, et al. CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells. STAR Protoc. 2021;2:100407 pubmed publisher
Sizova I, Kelterborn S, Verbenko V, Kateriya S, Hegemann P. Chlamydomonas POLQ is necessary for CRISPR/Cas9-mediated gene targeting. G3 (Bethesda). 2021;: pubmed publisher
Chen Z, Traniello I, Rana S, Cash Ahmed A, Sankey A, Yang C, et al. Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees. J Neurogenet. 2021;:1-13 pubmed publisher
Bastos de Oliveira M, Meier K, Jung S, Bartels Klein E, Coxam B, Geudens I, et al. Vasohibin-1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk. Development. 2021;: pubmed publisher
Zhao J, Inomata R, Kato Y, Miyagishi M. Development of aptamer-based inhibitors for CRISPR/Cas system. Nucleic Acids Res. 2020;: pubmed publisher
Lu S, Zhang Y, Yin H. Chimeric DNA-RNA Guide RNA Designs. Methods Mol Biol. 2021;2162:79-85 pubmed publisher
Saha C, Mohanraju P, Stubbs A, Dugar G, Hoogstrate Y, Kremers G, et al. Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. Sci Adv. 2020;6:eaaz4849 pubmed publisher
Yu Y, Guo Y, Tian Q, Lan Y, Yeh H, Zhang M, et al. An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms. Nat Chem Biol. 2020;16:387-390 pubmed publisher
Reuven N, Adler J, Broennimann K, Myers N, Shaul Y. Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019;9: pubmed publisher
Carmignotto G, Azzoni A. On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains. J Biotechnol. 2019;306:62-70 pubmed publisher
Kleinstiver B, Sousa A, Walton R, Tak Y, Hsu J, Clement K, et al. Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019;37:276-282 pubmed publisher
Murovec J, Guček K, Bohanec B, Avbelj M, Jerala R. DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes. Front Plant Sci. 2018;9:1594 pubmed publisher
Swinburne I, Mosaliganti K, Upadhyayula S, Liu T, Hildebrand D, Tsai T, et al. Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure. elife. 2018;7: pubmed publisher
Letelier J, de la Calle Mustienes E, Pieretti J, Naranjo S, Maeso I, Nakamura T, et al. A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins. Nat Genet. 2018;50:504-509 pubmed publisher
Ringer K, Roth M, Garey M, Piorczynski T, Suli A, Hansen J, et al. Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques. Cell Biol Int. 2018;42:849-858 pubmed publisher
Ocaña O, Coskun H, Minguillón C, Murawala P, Tanaka E, Galcerán J, et al. A right-handed signalling pathway drives heart looping in vertebrates. Nature. 2017;549:86-90 pubmed publisher
Mout R, Ray M, Yesilbag Tonga G, Lee Y, Tay T, Sasaki K, et al. Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing. ACS Nano. 2017;11:2452-2458 pubmed publisher
Thyme S, Akhmetova L, Montague T, Valen E, Schier A. Internal guide RNA interactions interfere with Cas9-mediated cleavage. Nat Commun. 2016;7:11750 pubmed publisher
Gagnon J, Valen E, Thyme S, Huang P, Akhmetova L, Ahkmetova L, et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE. 2014;9:e98186 pubmed publisher
product information
Catalog Number :
47327
Product Name :
pET-28b-Cas9-His
article :
doiPONE-D-14-14210 [pii]
id9908
pubmed_id24873830
bacterial resistance :
Kanamycin
cloning :
backbonepET-28b
backbone_mutation
backbone_origin
backbone_size5368
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
CRISPR
growth notes :
Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to express a His-tagged Cas9 protein in E. coli and purify it by nickel-based affinity purification.The Cas9 protein and sgRNA were incubated at room temperature to form the Cas9 protein/sgRNA complex in vitro, which was then microinjected into zebrafish zygotes. See publication for more details.
growth strain :
For in vitro expression and purification of Cas9 protein
growth temp :
For expression and purification of Cas9, transform into Rosetta strain.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NcoI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size4100
species
32644
Other
tags
locationC terminal on backbone
tagHis
resistance markers :
1666
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA