This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-Cas9
catalog :
47322
citations: 11
Reference
Hatzold J, Nett V, Brantsch S, Zhang J, Armistead J, Wessendorf H, et al. Matriptase-dependent epidermal pre-neoplasm in zebrafish embryos caused by a combination of hypotonic stress and epithelial polarity defects. PLoS Genet. 2023;19:e1010873 pubmed publisher
Halabi R, Cechmanek P, Hehr C, McFarlane S. Semaphorin3f as a cardiomyocyte derived regulator of heart chamber development. Cell Commun Signal. 2022;20:126 pubmed publisher
Blackwell D, Fraser S, Caluseriu O, Vivori C, Tyndall A, Lamont R, et al. Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo. G3 (Bethesda). 2022;12: pubmed publisher
Seleit A, Aulehla A, Paix A. Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach. elife. 2021;10: pubmed publisher
Halabi R, Watterston C, Hehr C, Mori Kreiner R, Childs S, McFarlane S. Semaphorin 3fa Controls Ocular Vascularization From the Embryo Through to the Adult. Invest Ophthalmol Vis Sci. 2021;62:21 pubmed publisher
Jeong C, Kang H, Hong S, Byeon E, Lee J, Lee Y, et al. Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma. Mar Pollut Bull. 2020;154:111038 pubmed publisher
Ahmed H, Hildebrand L, Wimmer E. Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii. BMC Biotechnol. 2019;19:85 pubmed publisher
Schwayer C, Shamipour S, Pranjic Ferscha K, Schauer A, Balda M, Tada M, et al. Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow. Cell. 2019;179:937-952.e18 pubmed publisher
Ashlin T, Blunsom N, Ghosh M, Cockcroft S, Rihel J. Pitpnc1a Regulates Zebrafish Sleep and Wake Behavior through Modulation of Insulin-like Growth Factor Signaling. Cell Rep. 2018;24:1389-1396 pubmed publisher
Cioni J, Wong H, Bressan D, Kodama L, Harris W, Holt C. Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. Neuron. 2018;97:1078-1093.e6 pubmed publisher
Gagnon J, Valen E, Thyme S, Huang P, Akhmetova L, Ahkmetova L, et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE. 2014;9:e98186 pubmed publisher
product information
Catalog Number :
47322
Product Name :
pCS2-Cas9
article :
doiPONE-D-14-14210 [pii]
id9908
pubmed_id24873830
bacterial resistance :
Ampicillin
cloning :
backbonepCS2
backbone_mutation
backbone_origin
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
Other
xenopus and zebrafish expression
growth notes :
Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
growth strain :
For in vivo expression of Cas9 or generation of Cas9 mRNA
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5ClaI
promoterSP6
sequencing_primer_3M13R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size4100
species
32644
Other
tags
resistance markers :
1666
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA