This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cas9m4-VP64
catalog :
47319
citations: 14
Reference
Weischedel J, Higgins L, Rogers S, Gramalla Schmitz A, Wyrzykowska P, Borgoni S, et al. Modular cytosine base editing promotes epigenomic and genomic modifications. Nucleic Acids Res. 2023;: pubmed publisher
Honda T, Nishikawa Y, Nishimura K, Teng D, Takemoto K, Ueda K. Effects of activation of the LINE-1 antisense promoter on the growth of cultured cells. Sci Rep. 2020;10:22136 pubmed publisher
Velazquez J, LeGraw R, Moghadam F, Tan Y, Kilbourne J, Maggiore J, et al. Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Cell Syst. 2021;12:41-55.e11 pubmed publisher
Böhm S, Splith V, Riedmayr L, Rötzer R, Gasparoni G, Nordström K, et al. A gene therapy for inherited blindness using dCas9-VPR-mediated transcriptional activation. Sci Adv. 2020;6:eaba5614 pubmed publisher
Colasante G, Qiu Y, Massimino L, Di Berardino C, Cornford J, Snowball A, et al. In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy. Brain. 2020;143:891-905 pubmed publisher
Colasante G, Lignani G, Brusco S, Di Berardino C, Carpenter J, Giannelli S, et al. dCas9-Based Scn1a Gene Activation Restores Inhibitory Interneuron Excitability and Attenuates Seizures in Dravet Syndrome Mice. Mol Ther. 2020;28:235-253 pubmed publisher
Chen H, Li Y, Du C, Li Y, Zhao J, Zheng X, et al. Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression. J Biotechnol. 2018;288:23-29 pubmed publisher
Williams R, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed A, et al. Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Development. 2018;145: pubmed publisher
Ferry Q, Lyutova R, Fulga T. Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Nat Commun. 2017;8:14633 pubmed publisher
Chavez A, Tuttle M, Pruitt B, Ewen Campen B, Chari R, Ter Ovanesyan D, et al. Comparison of Cas9 activators in multiple species. Nat Methods. 2016;13:563-567 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Chavez A, Scheiman J, Vora S, Pruitt B, Tuttle M, P R Iyer E, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12:326-8 pubmed publisher
Gao X, Tsang J, Gaba F, Wu D, Lu L, Liu P. Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Nucleic Acids Res. 2014;42:e155 pubmed publisher
Mali P, Aach J, Stranges P, Esvelt K, Moosburner M, Kosuri S, et al. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013;31:833-8 pubmed publisher
product information
Catalog Number :
47319
Product Name :
Cas9m4-VP64
article :
doi10.1038/nbt.2675
id7135
pubmed_id23907171
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3_TOPO
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
This plasmid contains a human codon optimized form of cas9.
growth strain :
Cas9m4 Activator
origin :
37
pi :
alt_names
cloning
entrez_gene
genbank_ids
mutation
nameCas9m4-VP64
shRNA_sequence
size
species
32644
Other
tags
resistance markers :
765
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA