This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-dCas9-VP64
catalog :
47107
citations: 21
Reference
Huang H, Zhang W, Zhang J, Zhao A, Jiang H. Epigenome editing based on CRISPR/dCas9p300 facilitates transdifferentiation of human fibroblasts into Leydig-like cells. Exp Cell Res. 2023;425:113551 pubmed publisher
Beyersdorf J, Bawage S, Iglesias N, Peck H, Hobbs R, Wroe J, et al. Robust, Durable Gene Activation In Vivo via mRNA-Encoded Activators. ACS Nano. 2022;: pubmed publisher
Ren C, Li H, Liu Y, Li S, Liang Z. Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators. Hortic Res. 2022;: pubmed publisher
Rappaport J, Entezari A, Caspi A, Caksa S, Jhaveri A, Stanek T, et al. A β-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer. Cell Mol Gastroenterol Hepatol. 2021;: pubmed publisher
Mózsik L, Pohl C, Meyer V, Bovenberg R, Nygård Y, Driessen A. Modular Synthetic Biology Toolkit for Filamentous Fungi. ACS Synth Biol. 2021;10:2850-2861 pubmed publisher
Weuring W, Dilevska I, Hoekman J, van de Vondervoort J, Koetsier M, van t Slot R, et al. CRISPRa-Mediated Upregulation of scn1laa During Early Development Causes Epileptiform Activity and dCas9-Associated Toxicity. CRISPR J. 2021;4:575-582 pubmed publisher
Jain S, Shukla S, Yang C, Zhang M, Fatma Z, Lingamaneni M, et al. TALEN outperforms Cas9 in editing heterochromatin target sites. Nat Commun. 2021;12:606 pubmed publisher
Polkoff K, Chung J, Simpson S, Gleason K, Piedrahita J. In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators. CRISPR J. 2020;3:409-418 pubmed publisher
Gamboa L, Phung E, Li H, Meyers J, Hart A, Miller I, et al. Heat-Triggered Remote Control of CRISPR-dCas9 for Tunable Transcriptional Modulation. ACS Chem Biol. 2020;15:533-542 pubmed publisher
Matharu N, Rattanasopha S, Tamura S, Maliskova L, Wang Y, Bernard A, et al. CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Science. 2019;363: pubmed publisher
Haenfler J, Skariah G, Rodriguez C, Monteiro da Rocha A, Parent J, Smith G, et al. Targeted Reactivation of FMR1 Transcription in Fragile X Syndrome Embryonic Stem Cells. Front Mol Neurosci. 2018;11:282 pubmed publisher
Pathak G, Spiltoir J, Höglund C, Polstein L, Heine Koskinen S, Gersbach C, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. 2017;45:e167 pubmed publisher
Bao Z, Jain S, Jaroenpuntaruk V, Zhao H. Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators. ACS Synth Biol. 2017;6:686-693 pubmed publisher
Brown A, Woods W, Perez Pinera P. Targeted Gene Activation Using RNA-Guided Nucleases. Methods Mol Biol. 2017;1468:235-50 pubmed publisher
Garcia Bloj B, Moses C, Sgro A, Plani Lam J, Arooj M, Duffy C, et al. Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Oncotarget. 2016;7:60535-60554 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Ji H, Jiang Z, Lu P, Ma L, Li C, Pan H, et al. Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter. Mol Ther. 2016;24:508-21 pubmed publisher
Limsirichai P, Gaj T, Schaffer D. CRISPR-mediated Activation of Latent HIV-1 Expression. Mol Ther. 2016;24:499-507 pubmed publisher
Hilton I, D Ippolito A, Vockley C, Thakore P, Crawford G, Reddy T, et al. Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015;33:510-7 pubmed publisher
Kabadi A, Gersbach C. Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods. 2014;69:188-97 pubmed publisher
Perez Pinera P, Kocak D, Vockley C, Adler A, Kabadi A, Polstein L, et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013;10:973-6 pubmed publisher
product information
Catalog Number :
47107
Product Name :
pcDNA-dCas9-VP64
article :
doi10.1038/nmeth.2600
id7189
pubmed_id23892895
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/
growth strain :
Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A, H840A (catalytically inactive)
namedCas9-VP64
shRNA_sequence
size4446
species
32644
Other
S. Pyogenes
tags
locationN terminal on insert
tagFlag
locationN terminal on insert
tagSV40 NLS
locationC terminal on insert
tagSV40 NLS
locationC terminal on insert
tagVP64
locationC terminal on insert
tagHA
resistance markers :
1264
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA