This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-dCas9
catalog :
47106
citations: 12
Reference
Raicu A, Castanheira P, Arnosti D. Retinoblastoma protein activity revealed by CRISPRi study of divergent Rbf1 and Rbf2 paralogs. bioRxiv. 2023;: pubmed publisher
Sgro A, Cursons J, Waryah C, Woodward E, Foroutan M, Lyu R, et al. Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma. Clin Epigenetics. 2023;15:73 pubmed publisher
Iorga A, Donovan K, Shojaie L, Johnson H, Kwok J, Suda J, et al. Interaction of RIPK1 and A20 modulates MAPK signaling in murine acetaminophen toxicity. J Biol Chem. 2021;:100300 pubmed publisher
Liu J, Sun M, Cho K, Gao X, Guo B. A CRISPR-Cas9 repressor for epigenetic silencing of KRAS. Pharmacol Res. 2021;164:105304 pubmed publisher
Dal Pra S, Hodgkinson C, Dzau V. Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient. PLoS ONE. 2019;14:e0223842 pubmed publisher
Chung J, Ain Q, Song Y, Yong S, Kim Y. Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance. Genome Res. 2019;29:1442-1452 pubmed publisher
Galonska C, Charlton J, Mattei A, Donaghey J, Clement K, Gu H, et al. Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun. 2018;9:597 pubmed publisher
Berlec A, Ć krlec K, Kocjan J, Olenic M, Strukelj B. Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis. Sci Rep. 2018;8:1009 pubmed publisher
Kleinjan D, Wardrope C, Nga Sou S, Rosser S. Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Nat Commun. 2017;8:1191 pubmed publisher
Garcia Bloj B, Moses C, Sgro A, Plani Lam J, Arooj M, Duffy C, et al. Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Oncotarget. 2016;7:60535-60554 pubmed publisher
Kabadi A, Gersbach C. Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods. 2014;69:188-97 pubmed publisher
Perez Pinera P, Kocak D, Vockley C, Adler A, Kabadi A, Polstein L, et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013;10:973-6 pubmed publisher
product information
Catalog Number :
47106
Product Name :
pcDNA-dCas9
article :
doi10.1038/nmeth.2600
id7189
pubmed_id23892895
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/
growth strain :
Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A, H840A (catalytically inactive)
namedCas9
shRNA_sequence
size4236
species
32644
Other
S. Pyogenes
tags
locationN terminal on insert
tagFlag
locationN terminal on insert
tagSV40 NLS
locationC terminal on insert
tagSV40 NLS
resistance markers :
1264
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA