This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-puro-Bcl2
catalog :
46971
citations: 9
Reference
Nardi F, Perurena N, SCHADE A, Li Z, Ngo K, Ivanova E, et al. Cotargeting a MYC/eIF4A-survival axis improves the efficacy of KRAS inhibitors in lung cancer. J Clin Invest. 2023;133: pubmed publisher
Moore L, Holmes D, Sharma A, Landazuri Vinueza J, Lagunoff M. Bcl-xL is required to protect endothelial cells latently infected with KSHV from virus induced intrinsic apoptosis. PLoS Pathog. 2023;19:e1011385 pubmed publisher
Pourzia A, Olson M, Bailey S, Boroughs A, Aryal A, Ryan J, et al. Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells. Cell Death Dis. 2023;14:267 pubmed publisher
Mathias T, Ju J, Lee R, Thompson K, Mull M, Annis D, et al. Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling. Cancers (Basel). 2022;14: pubmed publisher
Yin L, Zeng Y, Zeng R, Chen Y, Wang T, Rodabaugh K, et al. Protein kinase RNA-activated controls mitotic progression and determines paclitaxel chemosensitivity through B-cell lymphoma 2 in ovarian cancer. Oncogene. 2021;40:6772-6785 pubmed publisher
Larrue C, Guiraud N, Mouchel P, Dubois M, Farge T, Gotanègre M, et al. Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells. Nat Commun. 2021;12:422 pubmed publisher
Bester A, Lee J, Chavez A, Lee Y, Nachmani D, Vora S, et al. An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance. Cell. 2018;173:649-664.e20 pubmed publisher
Kozaki T, Komano J, Kanbayashi D, Takahama M, Misawa T, Satoh T, et al. Mitochondrial damage elicits a TCDD-inducible poly(ADP-ribose) polymerase-mediated antiviral response. Proc Natl Acad Sci U S A. 2017;114:2681-2686 pubmed publisher
Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce L, et al. Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013;19:1748-59 pubmed publisher
product information
Catalog Number :
46971
Product Name :
pCDH-puro-Bcl2
article :
doi10.1158/1078-0432.CCR-12-3066
id7005
pubmed_id23403638
bacterial resistance :
Ampicillin
cloning :
backbonePCDH-CMV-MCS-EF1-PURO
backbone_mutation
backbone_originSystem Biosciences
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Bcl-2 was cut from 3544 pMIG Bcl-2 (Addgene #8793) and subcloned into the EcoRI sites of pCDH-CMV-MCS-EF1-Puro.
growth strain :
lentiviral expression of Bcl2
origin :
37
pi :
alt_names
B-cell CLL/lymphoma 2_
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBcl-2, PPP1R50
geneBCL2
id596
genbank_ids
mutation
nameBcl2
shRNA_sequence
size720
species
9606
Homo sapiens
tags
resistance markers :
1651
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA