This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-puro-myr-HA-Akt1
catalog :
46969
citations: 13
Reference
Lv X, Lu X, Cao J, Luo Q, Ding Y, Peng F, et al. Modulation of the proteostasis network promotes tumor resistance to oncogenic KRAS inhibitors. Science. 2023;381:eabn4180 pubmed publisher
Wei C, Du J, Shen Y, Wang Z, Lin Q, Chen J, et al. Anticancer effect of involucrasin A on colorectal cancer cells by modulating the Akt/MDM2/p53 pathway. Oncol Lett. 2023;25:218 pubmed publisher
Satta T, Li L, Chalasani S, Hu X, Nkwocha J, Sharma K, et al. Dual mTORC1/2 Inhibition Synergistically Enhances AML Cell Death in Combination with the BCL2 Antagonist Venetoclax. Clin Cancer Res. 2023;29:1332-1343 pubmed publisher
Wang Z, Zuo J, Zhang L, Zhang Z, Wei Y. Plantamajoside promotes metformin-induced apoptosis, autophagy and proliferation arrest of liver cancer cells via suppressing Akt/GSK3β signaling. Hum Exp Toxicol. 2022;41:9603271221078868 pubmed publisher
Rashkovan M, Albero R, Gianni F, Pérez Durán P, Miller H, Mackey A, et al. Intracellular cholesterol pools regulate oncogenic signaling and epigenetic circuitries in Early T-cell Precursor Acute Lymphoblastic Leukemia. Cancer Discov. 2021;: pubmed publisher
Huang N, Li S, Xie Y, Han Q, Xu X, Sheng Z. Reprogramming an energetic AKT-PAK5 axis boosts axon energy supply and facilitates neuron survival and regeneration after injury and ischemia. Curr Biol. 2021;31:3098-3114.e7 pubmed publisher
Li T, Wang X, Ju E, da Silva S, Chen L, Zhang X, et al. RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation. Nat Commun. 2021;12:1055 pubmed publisher
Shi Y, He Y, Zhou Y, Li H, Yang D, Li R, et al. LSD1 negatively regulates autophagy in myoblast cells by driving PTEN degradation. Biochem Biophys Res Commun. 2019;: pubmed publisher
Yu V, Ko J, Ning L, Dai W, Law S, Lung M. Endoplasmic reticulum-localized ECM1b suppresses tumor growth and regulates MYC and MTORC1 through modulating MTORC2 activation in esophageal squamous cell carcinoma. Cancer Lett. 2019;461:56-64 pubmed publisher
Hornsveld M, Smits L, Meerlo M, van Amersfoort M, Groot Koerkamp M, van Leenen D, et al. FOXO Transcription Factors Both Suppress and Support Breast Cancer Progression. Cancer Res. 2018;78:2356-2369 pubmed publisher
Taylor C, Mannion D, Miranda F, KaramiNejadRanjbar M, Herrero González S, Hellner K, et al. Loss of PFKFB4 induces cell death in mitotically arrested ovarian cancer cells. Oncotarget. 2017;8:17960-17980 pubmed publisher
Cheng Y, Zhao G, Zhang S, Nigim F, Zhou G, Yu Z, et al. AS1411-Induced Growth Inhibition of Glioma Cells by Up-Regulation of p53 and Down-Regulation of Bcl-2 and Akt1 via Nucleolin. PLoS ONE. 2016;11:e0167094 pubmed publisher
Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce L, et al. Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013;19:1748-59 pubmed publisher
product information
Catalog Number :
46969
Product Name :
pCDH-puro-myr-HA-Akt1
article :
doi10.1158/1078-0432.CCR-12-3066
id7005
pubmed_id23403638
bacterial resistance :
Ampicillin
cloning :
backbonePCDH-CMV-MCS-EF1-PURO
backbone_mutation
backbone_originSystem Biosciences
backbone_size7384
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Myr-HA-Akt1 was amplified by PCR from pcDNA3-HA-Myr-Akt1 (Addgene plasmid #9008) and subcloned into the XbaI and EcoRI sites of the pCDH-CMV-MCS-EF1-Puro lentiviral vector.
growth strain :
lentiviral expression of myr-HA-Akt1
origin :
37
pi :
alt_names
v-akt murine thymoma viral oncogene homolog 1__
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XbaI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAKT1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmyr
locationN terminal on insert
tagHA
resistance markers :
1651
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA