This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pK7WGF2::hCas9
catalog :
46965
citations: 4
Reference
Chen L, Li W, Katin Grazzini L, Ding J, Gu X, Li Y, et al. A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants. Hortic Res. 2018;5:13 pubmed publisher
Alagoz Y, Gurkok T, Zhang B, Unver T. Manipulating the Biosynthesis of Bioactive Compound Alkaloids for Next-Generation Metabolic Engineering in Opium Poppy Using CRISPR-Cas 9 Genome Editing Technology. Sci Rep. 2016;6:30910 pubmed publisher
Zhang B, Yang X, Yang C, Li M, Guo Y. Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia. Sci Rep. 2016;6:20315 pubmed publisher
Nekrasov V, Staskawicz B, Weigel D, Jones J, Kamoun S. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013;31:691-3 pubmed publisher
product information
Catalog Number :
46965
Product Name :
pK7WGF2::hCas9
article :
doi10.1038/nbt.2655
id7238
pubmed_id23929340
bacterial resistance :
Spectinomycin
cloning :
backbonepK7WGF2
backbone_mutation
backbone_origin
backbone_size10257
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Other
Plant expression
growth notes :
For more information on Kamoun Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kamoun/
growth strain :
Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter35S
sequencing_primer_3TTGTACAAGAAAGCTGGGTCG
sequencing_primer_5CAGGCTCCGCGGCCGC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namehCas9
shRNA_sequence
size4140
species
100
Synthetic
tags
locationN terminal on insert
tageGFP
plasmid copy :
Addgene Plasmid 41815: hCas9
resistance markers :
1659
tags :
Low Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA