This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pICE-EGFP-FLAG-Ku70siR-Mut6E
catalog :
46962
citations: 1
Reference
Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed publisher
product information
Catalog Number :
46962
Product Name :
pICE-EGFP-FLAG-Ku70siR-Mut6E
article :
doi10.1083/jcb.201303073
id7121
pubmed_id23897892
bacterial resistance :
Ampicillin
cloning :
backbonepICE
backbone_mutation
backbone_origin
backbone_size5044
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
origin :
37
pi :
alt_names
XRCC6
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5HindIII
promoterCMV Tet
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5GTAGGCGTGTACGGTGGGAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCTC75, CTCBF, G22P1, KU70, ML8, TLAA
geneXRCC6
id2547
genbank_ids
NM_001469.3
mutationMut6E corresponding to K282E K287E T300E K331E K338E R403E
nameKu70
shRNA_sequence
size1830
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP-FLAG
resistance markers :
1650
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA