This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1-FLAG-Ku80
catalog :
46958
citations: 10
Reference
Müller B, Serafin F, Laucke L, Rheinhard W, Wimmer T, Stieger K. Characterization of Double-Strand Break Repair Protein Ku80 Location Within the Murine Retina. Invest Ophthalmol Vis Sci. 2022;63:22 pubmed publisher
Demirbag Sarikaya S, Akkoç Y, Turgut S, Erbil Bilir S, Kocaturk N, Dengjel J, et al. A novel ATG5 interaction with Ku70 potentiates DNA repair upon genotoxic stress. Sci Rep. 2022;12:8134 pubmed publisher
Levone B, Lombardi S, Barabino S. Laser microirradiation as a tool to investigate the role of liquid-liquid phase separation in DNA damage repair. STAR Protoc. 2022;3:101146 pubmed publisher
Levone B, Lenzken S, Antonaci M, Maiser A, Rapp A, Conte F, et al. FUS-dependent liquid-liquid phase separation is important for DNA repair initiation. J Cell Biol. 2021;220: pubmed publisher
Wang Y, Fu Z, Li X, Liang Y, Pei S, Hao S, et al. Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. Immunity. 2021;54:632-647.e9 pubmed publisher
Onn L, Portillo M, Ilic S, Cleitman G, Stein D, Kaluski S, et al. SIRT6 is a DNA double-strand break sensor. elife. 2020;9: pubmed publisher
Abbotts R, Topper M, Biondi C, Fontaine D, Goswami R, Stojanovic L, et al. DNA methyltransferase inhibitors induce a BRCAness phenotype that sensitizes NSCLC to PARP inhibitor and ionizing radiation. Proc Natl Acad Sci U S A. 2019;116:22609-22618 pubmed publisher
Ha G, Ji J, Chae S, Park J, Kim S, Lee J, et al. Pellino1 regulates reversible ATM activation via NBS1 ubiquitination at DNA double-strand breaks. Nat Commun. 2019;10:1577 pubmed publisher
Fouquin A, Guirouilh Barbat J, Lopez B, Hall J, Amor Gueret M, Pennaneach V. PARP2 controls double-strand break repair pathway choice by limiting 53BP1 accumulation at DNA damage sites and promoting end-resection. Nucleic Acids Res. 2017;45:12325-12339 pubmed publisher
Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed publisher
product information
Catalog Number :
46958
Product Name :
pEGFP-C1-FLAG-Ku80
article :
doi10.1083/jcb.201303073
id7121
pubmed_id23897892
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1-FLAG
backbone_mutation
backbone_origin
backbone_size4766
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The depositor notes that the D158G point mutation does not affect plasmid function.
growth strain :
Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.
origin :
37
pi :
alt_names
XRCC5
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3GCAAGTAAAACCTCTACAAATGTGG
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKARP-1, KARP1, KU80, KUB2, Ku86, NFIV
geneXRCC5
id7520
genbank_ids
NM_021141.3
mutation
nameKu80
shRNA_sequence
size2199
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP-FLAG
resistance markers :
1650
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA