This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1-FLAG-Ku70
catalog :
46957
citations: 12
Reference
Saleh H, Liloglou T, Rigden D, Parsons J, Grundy G. KH-like Domains in PARP9/DTX3L and PARP14 Coordinate Protein-Protein Interactions to Promote Cancer Cell Survival. J Mol Biol. 2024;436:168434 pubmed publisher
Demirbag Sarikaya S, Akkoç Y, Turgut S, Erbil Bilir S, Kocaturk N, Dengjel J, et al. A novel ATG5 interaction with Ku70 potentiates DNA repair upon genotoxic stress. Sci Rep. 2022;12:8134 pubmed publisher
Wang Y, Ho T, Hariharan A, Goh H, Wong Y, Verkaik N, et al. Rapid recruitment of p53 to DNA damage sites directs DNA repair choice and integrity. Proc Natl Acad Sci U S A. 2022;119:e2113233119 pubmed publisher
Tanaka H, Kondo K, Fujita K, Homma H, Tagawa K, Jin X, et al. HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology. Commun Biol. 2021;4:1175 pubmed publisher
Wang Y, Fu Z, Li X, Liang Y, Pei S, Hao S, et al. Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. Immunity. 2021;54:632-647.e9 pubmed publisher
Sharma A, Alswillah T, Kapoor I, Debjani P, Willard B, Summers M, et al. USP14 is a deubiquitinase for Ku70 and critical determinant of non-homologous end joining repair in autophagy and PTEN-deficient cells. Nucleic Acids Res. 2020;48:736-747 pubmed publisher
Nicolai S, Mahen R, Raschellà G, Marini A, Pieraccioli M, Malewicz M, et al. ZNF281 is recruited on DNA breaks to facilitate DNA repair by non-homologous end joining. Oncogene. 2020;39:754-766 pubmed publisher
Parvin S, Ramírez Labrada A, Aumann S, Lu X, Weich N, Santiago G, et al. LMO2 Confers Synthetic Lethality to PARP Inhibition in DLBCL. Cancer Cell. 2019;36:237-249.e6 pubmed publisher
Rushing A, Hoang K, Polakowski N, Lemasson I. The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining. J Virol. 2018;92: pubmed publisher
Fouquin A, Guirouilh Barbat J, Lopez B, Hall J, Amor Gueret M, Pennaneach V. PARP2 controls double-strand break repair pathway choice by limiting 53BP1 accumulation at DNA damage sites and promoting end-resection. Nucleic Acids Res. 2017;45:12325-12339 pubmed publisher
Frost J, Olanubi O, Cheng S, Soriano A, Crisostomo L, Lopez A, et al. The interaction of adenovirus E1A with the mammalian protein Ku70/XRCC6. Virology. 2017;500:11-21 pubmed publisher
Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed publisher
product information
Catalog Number :
46957
Product Name :
pEGFP-C1-FLAG-Ku70
article :
doi10.1083/jcb.201303073
id7121
pubmed_id23897892
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1-FLAG
backbone_mutationInserted a FLAG tag and XhoI and NotI unique restriction sites between BglII and HindIII.
backbone_origin
backbone_size4766
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.
origin :
37
pi :
alt_names
XRCC6
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3GCAAGTAAAACCTCTACAAATGTGG
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCTC75, CTCBF, G22P1, KU70, ML8, TLAA
geneXRCC6
id2547
genbank_ids
NM_001469.3
mutation
nameKu70
shRNA_sequence
size1830
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP-FLAG
resistance markers :
1650
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA