This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
5HRE/GFP
catalog :
46926
citations: 17
Reference
Matsuoka T, Sugiyama A, Miyawaki Y, Hidaka Y, Okuno Y, Sakai H, et al. Newly developed preclinical models reveal broad-spectrum CDK inhibitors as potent drugs for CRPC exhibiting primary resistance to enzalutamide. Cancer Sci. 2023;: pubmed publisher
Samuel T, Rapic S, O Brien C, Edson M, Zhong Y, Dacosta R. Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse model. Sci Adv. 2023;9:eade8672 pubmed publisher
Sitte Z, Larson T, McIntosh J, Sinanian M, Lockett M. Selecting the appropriate indirect viability assay for 3D paper-based cultures: a data-driven study. Analyst. 2023;148:2245-2255 pubmed publisher
Zou J, Wang W, Lu Y, Ayala J, Dong K, Zhou H, et al. Neddylation is required for perinatal cardiac development through stimulation of metabolic maturation. Cell Rep. 2023;42:112018 pubmed publisher
Zeh N, Bräuer M, Raab N, Handrick R, Otte K. Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control. Sci Rep. 2022;12:2268 pubmed publisher
Mallet C, Cochard J, Leclercq S, Trapp Fragnet L, Chouteau P, Denesvre C. Hypoxia and HIF-1 Trigger Marek's Disease Virus Reactivation in Lymphoma-Derived Latently Infected T Lymphocytes. J Virol. 2021;:JVI0142721 pubmed publisher
Janská L, Anandi L, Kirchberger N, Marinkovic Z, Schachtner L, Guzelsoy G, et al. The MEMIC is an ex vivo system to model the complexity of the tumor microenvironment. Dis Model Mech. 2021;14: pubmed publisher
Zeh N, Schlossbauer P, Raab N, Klingler F, Handrick R, Otte K. Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity. Metab Eng Commun. 2021;13:e00181 pubmed publisher
Ayuso J, Rehman S, Farooqui M, Virumbrales Muñoz M, Setaluri V, Skala M, et al. Microfluidic Tumor-on-a-Chip Model to Study Tumor Metabolic Vulnerability. Int J Mol Sci. 2020;21: pubmed publisher
Boyce M, Simke W, Kenney R, Lockett M. Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs). Anal Methods. 2020;12:18-24 pubmed publisher
Park J, Kim M, Kim W, Kwon K, Ha K, Choi B, et al. Isolinderalactone suppresses human glioblastoma growth and angiogenic activity in 3D microfluidic chip and in vivo mouse models. Cancer Lett. 2020;478:71-81 pubmed publisher
Tan H, Tan S. The focal adhesion protein kindlin-2 controls mitotic spindle assembly by inhibiting histone deacetylase 6 and maintaining α-tubulin acetylation. J Biol Chem. 2020;295:5928-5943 pubmed publisher
Duette G, Pereyra Gerber P, Rubione J, Pérez P, Landay A, Crowe S, et al. Induction of HIF-1α by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation. MBio. 2018;9: pubmed publisher
Kim H, Lin Q, Glazer P, Yun Z. The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells. Breast Cancer Res. 2018;20:16 pubmed publisher
Xia J, Wu H, Lai B, Zheng L, Zhou D, Chang Z, et al. Gene delivery of hypoxia-inducible VEGF targeting collagen effectively improves cardiac function after myocardial infarction. Sci Rep. 2017;7:13273 pubmed publisher
Truong A, Lockett M. Oxygen as a chemoattractant: confirming cellular hypoxia in paper-based invasion assays. Analyst. 2016;141:3874-82 pubmed publisher
Vordermark D, Shibata T, Brown J. Green fluorescent protein is a suitable reporter of tumor hypoxia despite an oxygen requirement for chromophore formation. Neoplasia. 2001;3:527-34 pubmed
product information
Catalog Number :
46926
Product Name :
5HRE/GFP
article :
doi10.1038/sj/neo/7900192
id6991
pubmed_id11774035
bacterial resistance :
Ampicillin
cloning :
backbonepEF/myc/cyto
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The 35-bp human VEGF HRE sequence is: CCACAGTGCATACGTGGGCTCCAACAGGTCCTCTT The d2EGFP fragment was amplified from a Clontech (Palo Alto, CA) vector and encodes a destabilized red- shifted variant of wild-type GFP with an excitation maximum of 488 nm, an emission maximum of 507 nm, and a half-life of approximately 2 hours.
growth strain :
hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system
origin :
37
pi :
alt_names
VEGFA
VEGF
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoterEF-1 promoter
sequencing_primer_3BGHrev
sequencing_primer_5EF1a-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesL-VEGF, MVCD1, VEGF, VPF
geneVEGFA
id7422
genbank_ids
mutationfive copies of a 35-bp fragment from the hypoxia-responsive element (HRE) of the human VEGF gene and a human cytomegalovirus minimal promoter
name5X HRE of VEGF
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagd2EGFP
resistance markers :
1644
1645
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA