This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YFP-Parkin C431N
catalog :
46924
citations: 3
Reference
Ansari M, Khan N, Ahmad I, Haqqi T. Parkin clearance of dysfunctional mitochondria regulates ROS levels and increases survival of human chondrocytes. Osteoarthritis Cartilage. 2018;26:1087-1097 pubmed publisher
Brennan L, Khoury J, Kantorow M. Parkin elimination of mitochondria is important for maintenance of lens epithelial cell ROS levels and survival upon oxidative stress exposure. Biochim Biophys Acta Mol Basis Dis. 2017;1863:21-32 pubmed publisher
Lazarou M, Narendra D, Jin S, Tekle E, Banerjee S, Youle R. PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding. J Cell Biol. 2013;200:163-72 pubmed publisher
product information
Catalog Number :
46924
Product Name :
YFP-Parkin C431N
article :
doi10.1083/jcb.201210111
id6990
pubmed_id23319602
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-C1
backbone_mutation
backbone_originClontech
backbone_size4698
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses mutant Parkin with YFP tag in mammalian cells
origin :
37
pi :
alt_names
Parkin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3CTACAAATGTGGTATGGCTG
sequencing_primer_5AGAAGCGCGATCACATGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562
mutationchanged cysteine 431 to asparagine
namePark2
shRNA_sequence
size1398
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEYFP
resistance markers :
604
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA