This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSNR52-sgTEF1
catalog :
46922
citations: 1
Reference
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher
product information
Catalog Number :
46922
Product Name :
pSNR52-sgTEF1
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backboneAH057
backbone_mutation
backbone_origin
backbone_size5083
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
growth notes :
gRNA target sequence TTGATATTTAAGTTAATAAA For more information on Qi and Wiessman Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Qi-weissman/ and http://www.addgene.org/crispr/qi/
growth strain :
Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterSNR52
sequencing_primer_3
sequencing_primer_5gcgattaagttgggtaacg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPR080W
geneTEF1
id856195
genbank_ids
mutation
namesgTEF1 promoter
shRNA_sequence
size119
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
1487
1648
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA