pTDH3-dCas9-Mxi1 | Addgene 46921 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTDH3-dCas9-Mxi1

catalog :

46921

citations: 8

Reference
Zhang Y, Zheng L, Xie K. CRISPR/dCas9-Mediated Gene Silencing in Two Plant Fungal Pathogens. mSphere. 2023;8:e0059422 pubmed publisher
Presnell K, Melhem O, Morse N, Alper H. Modular, Synthetic Boolean Logic Gates Enabled in Saccharomyces cerevisiae through T7 Polymerases/CRISPR dCas9 Designs. ACS Synth Biol. 2022;11:3414-3425 pubmed publisher
Lim S, Baek J, Jeon B, Seo J, Kim M, Byun J, et al. CRISPRi-Guided Metabolic Flux Engineering for Enhanced Protopanaxadiol Production in Saccharomyces cerevisiae. Int J Mol Sci. 2021;22: pubmed publisher
Li P, Fu X, Zhang L, Li S. CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance. Microb Biotechnol. 2018;: pubmed publisher
Jensen E, Ferreira R, Jakočiūnas T, Arsovska D, Zhang J, Ding L, et al. Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies. Microb Cell Fact. 2017;16:46 pubmed publisher
Deaner M, Alper H. Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae. Metab Eng. 2017;40:14-22 pubmed publisher
Jusiak B, Cleto S, Perez PiÃ±era P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher

product information

Catalog Number :

46921

Product Name :

pTDH3-dCas9-Mxi1

article :

doi	10.1016/j.cell.2013.06.044
id	7026
pubmed_id	23849981

bacterial resistance :

Ampicillin

cloning :

backbone

pJED103

backbone_mutation

backbone_origin

backbone_size

6900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

CRISPR

growth notes :

For more information on Qi and Wiessman Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Qi-weissman/ and http://www.addgene.org/crispr/qi/ Please note, dCas9 contains an N612K mutation that does not affect function.

growth strain :

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	TDH3
sequencing_primer_3
sequencing_primer_5	gGAGCTCactcgGAATTCagtctACTAGT
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

dCas9-Mxi1

shRNA_sequence

size

4365

species