This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTDH3-dCas9
catalog :
46920
citations: 8
Reference
Nova x10d i x107 A, Men xe9 ndez D, Ljubas J, Barbari x107 S, Stutz F, Soudet J, et al. Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure. PLoS Genet. 2022;18:e1010432 pubmed publisher
Presnell K, Melhem O, Morse N, Alper H. Modular, Synthetic Boolean Logic Gates Enabled in Saccharomyces cerevisiae through T7 Polymerases/CRISPR dCas9 Designs. ACS Synth Biol. 2022;11:3414-3425 pubmed publisher
Soudet J, Beyrouthy N, Pastucha A, Maffioletti A, Menéndez D, Bakir Z, et al. Antisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone. Nucleic Acids Res. 2022;: pubmed publisher
Cámara E, Lenitz I, Nygård Y. A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12. Sci Rep. 2020;10:14605 pubmed publisher
Wurihan W, Huang Y, Weber A, Wu X, Fan H. Nonspecific toxicities of Streptococcus pyogenes and Staphylococcus aureus dCas9 in Chlamydia trachomatis. Pathog Dis. 2019;77: pubmed publisher
Howe F, Russell A, Lamstaes A, El Sagheer A, Nair A, Brown T, et al. CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape. elife. 2017;6: pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher
product information
Catalog Number :
46920
Product Name :
pTDH3-dCas9
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backbonepJED103
backbone_mutation
backbone_origin
backbone_size6900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
growth notes :
The Cas9 contains a N612K mutation that does not effect the function of the enzyme. For more information on Qi and Wiessman Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Qi-weissman/ and http://www.addgene.org/crispr/qi/
growth strain :
Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterTDH3
sequencing_primer_3
sequencing_primer_5gGAGCTCactcgGAATTCagtctACTAGT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9
shRNA_sequence
size4135
species
tags
resistance markers :
1487
1648
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA