This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pU6-sgCXCR4-2
catalog :
46917
citations: 4
Reference
Hickey K, Dickson K, Cogan J, Replogle J, Schoof M, D Orazio K, et al. GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control. Mol Cell. 2020;79:950-962.e6 pubmed publisher
Jost M, Santos D, Saunders R, Horlbeck M, Hawkins J, Scaria S, et al. Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs. Nat Biotechnol. 2020;38:355-364 pubmed publisher
Zaini M, Patel S, Syafruddin S, Rodrigues P, Vanharanta S. Endogenous HIF2A reporter systems for high-throughput functional screening. Sci Rep. 2018;8:12063 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher
product information
Catalog Number :
46917
Product Name :
pU6-sgCXCR4-2
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backbonepSICO derivative
backbone_mutation
backbone_originAddgene
backbone_size8200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
gRNA target sequence GCAGGTAGCAAAGTGACGCCGA For more information on Qi and Wiessman Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Qi-weissman/ and http://www.addgene.org/crispr/qi/
growth strain :
Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5BstX1
promoterU6
sequencing_primer_3
sequencing_primer_5cttgtgggagaagctcggcta
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1
geneCXCR4
id7852
genbank_ids
mutation
namesgCXCR4 -2
shRNA_sequence
size103
species
9606
Homo sapiens
tags
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5caccccattgacgtcaatggg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePuromycin resistance and mCherry
shRNA_sequence
size1377
species
tags
resistance markers :
1487
1648
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA