pMSCV-LTR-dCas9-p65AD-BFP | Addgene 46913 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMSCV-LTR-dCas9-p65AD-BFP

catalog :

46913

citations: 3

Reference
Jusiak B, Cleto S, Perez PiÃ±era P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, et al. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015;5:16277 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher

product information

Catalog Number :

46913

Product Name :

pMSCV-LTR-dCas9-p65AD-BFP

article :

doi	10.1016/j.cell.2013.06.044
id	7026
pubmed_id	23849981

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV-puro

backbone_mutation

backbone_origin

Clontech

backbone_size

6300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

CRISPR

growth strain :

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	LTR
sequencing_primer_3	GAGACGTGCTACTTCCATTTGTC
sequencing_primer_5	CCCTTGAACCTCCTCGTTCGACC
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

dCas9-p65AD-BFP fusion

shRNA_sequence

size

5670

species

9606

Homo sapiens