This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV-LTR-dCas9-VP64-BFP
catalog :
46912
citations: 11
Reference
Feser C, Lees C, Lammers D, Riddle M, Bingham J, Eckert M, et al. Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production. Int J Mol Sci. 2022;23: pubmed publisher
Morandell J, Schwarz L, Basilico B, Tasciyan S, Dimchev G, Nicolas A, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nat Commun. 2021;12:3058 pubmed publisher
Alexander P, Kucera G, Pladna K, Pardee T. The Novel Phospholipid Mimetic KPC34 Is Highly Active Against Acute Myeloid Leukemia with Activated Protein Kinase C. Transl Oncol. 2020;13:100780 pubmed publisher
Zhang Y, Arango G, Li F, Xiao X, Putatunda R, Yu J, et al. Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling. BMC Med Genomics. 2018;11:78 pubmed publisher
Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, et al. Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Nucleic Acids Res. 2018;46:e25 pubmed publisher
Suzuki K, Tsunekawa Y, Hernández Benítez R, Wu J, Zhu J, Kim E, et al. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature. 2016;540:144-149 pubmed publisher
Nguyen D, Miyaoka Y, Gilbert L, Mayerl S, Lee B, Weissman J, et al. Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity. Nat Commun. 2016;7:12009 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, et al. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015;5:16277 pubmed publisher
Gao X, Tsang J, Gaba F, Wu D, Lu L, Liu P. Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Nucleic Acids Res. 2014;42:e155 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher
product information
Catalog Number :
46912
Product Name :
pMSCV-LTR-dCas9-VP64-BFP
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-puro
backbone_mutation
backbone_originClontech
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
CRISPR
growth notes :
The Cas9 contains a N612K mutation that does not effect the function of the enzyme.
growth strain :
Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterLTR
sequencing_primer_3GAGACGTGCTACTTCCATTTGTC
sequencing_primer_5CCCTTGAACCTCCTCGTTCGACC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9-VP64-BFP fusion
shRNA_sequence
size5055
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xNLS
locationC terminal on insert
tagBFP
locationC terminal on insert
tagVP64 domain
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3
sequencing_primer_5CGGGCGCCCGAAGGTCCTCCG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePuromycin resistance
shRNA_sequence
size654
species
tags
resistance markers :
1487
1648
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA