This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHR-SFFV-dCas9-BFP-KRAB
catalog :
46911
citations: 84
Reference
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Rinzema N, Sofiadis K, Tjalsma S, Verstegen M, Oz Y, Valdes Quezada C, et al. Building regulatory landscapes reveals that an enhancer can recruit cohesin to create contact domains, engage CTCF sites and activate distant genes. Nat Struct Mol Biol. 2022;29:563-574 pubmed publisher
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Joung J, Kirchgatterer P, Singh A, Cho J, Nety S, Larson R, et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun. 2022;13:1606 pubmed publisher
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Praznik A, Fink T, Franko N, Lonzarić J, Bencina M, Jerala N, et al. Regulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage. Nat Commun. 2022;13:1323 pubmed publisher
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Schmidt R, Steinhart Z, Layeghi M, Freimer J, Bueno R, Nguyen V, et al. CRISPR activation and interference screens decode stimulation responses in primary human T cells. Science. 2022;375:eabj4008 pubmed publisher
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Aronson B, Scourzic L, Shah V, Swanzey E, Kloetgen A, Polyzos A, et al. A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus. Dev Cell. 2021;56:3052-3065.e5 pubmed publisher
Chen P, Hussmann J, Yan J, Knipping F, Ravisankar P, Chen P, et al. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell. 2021;184:5635-5652.e29 pubmed publisher
Gandelman M, Dansithong W, Kales S, Paul S, Maag G, Aoyama E, et al. The AKT modulator A-443654 reduces α-synuclein expression and normalizes ER stress and autophagy. J Biol Chem. 2021;297:101191 pubmed publisher
Morelli E, Gullà A, Amodio N, Taiana E, Neri A, Fulciniti M, et al. CRISPR Interference (CRISPRi) and CRISPR Activation (CRISPRa) to Explore the Oncogenic lncRNA Network. Methods Mol Biol. 2021;2348:189-204 pubmed publisher
Das S, Chadwick B. CRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy. Sci Rep. 2021;11:12598 pubmed publisher
Chu C, Sjöström M, Egusa E, Gibb E, Badura M, Zhu J, et al. Heterogeneity in NECTIN4 expression across molecular subtypes of urothelial cancer mediates sensitivity to enfortumab vedotin. Clin Cancer Res. 2021;: pubmed publisher
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Attenello F, Tsung K, Bishara I, Loh Y, Chen T. In vivo CRISPR screening for novel noncoding RNA functional targets in glioblastoma models. J Neurosci Res. 2021;99:2029-2045 pubmed publisher
Marx N, Dhiman H, Schmieder V, Freire C, Nguyen L, Klanert G, et al. Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Metab Eng. 2021;66:268-282 pubmed publisher
Brackett K, Mungale A, Lopez Isidro M, Proctor D, Najarro G, Arias C. CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi's Sarcoma-Associated Herpesvirus-Infected Cells. Viruses. 2021;13: pubmed publisher
Zhu G, Luo H, Feng Y, Guryanova O, Xu J, Chen S, et al. HOXBLINC long non-coding RNA activation promotes leukemogenesis in NPM1-mutant acute myeloid leukemia. Nat Commun. 2021;12:1956 pubmed publisher
Zhang Y, Fang D. The incorporation loci of H3.3K36M determine its preferential prevalence in chondroblastomas. Cell Death Dis. 2021;12:311 pubmed publisher
Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu J, et al. Transcription factor competition at the γ-globin promoters controls hemoglobin switching. Nat Genet. 2021;53:511-520 pubmed publisher
Huh D, Passarelli M, Gao J, Dusmatova S, Goin C, Fish L, et al. A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. EMBO J. 2021;40:e106696 pubmed publisher
Liu S, Horlbeck M, Weissman J, Lim D. Genome-Scale Perturbation of Long Noncoding RNA Expression Using CRISPR Interference. Methods Mol Biol. 2021;2254:323-338 pubmed publisher
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Batra R, Nelles D, Roth D, Krach F, Nutter C, Tadokoro T, et al. The sustained expression of Cas9 targeting toxic RNAs reverses disease phenotypes in mouse models of myotonic dystrophy type 1. Nat Biomed Eng. 2020;: pubmed publisher
Beesley J, Sivakumaran H, Moradi Marjaneh M, Shi W, Hillman K, Kaufmann S, et al. eQTL Colocalization Analyses Identify NTN4 as a Candidate Breast Cancer Risk Gene. Am J Hum Genet. 2020;107:778-787 pubmed publisher
See K, Kiseleva A, Smith C, Liu F, Li J, Poleshko A, et al. Histone methyltransferase activity programs nuclear peripheral genome positioning. Dev Biol. 2020;466:90-98 pubmed publisher
Stojic L. Tuning the Expression of Long Noncoding RNA Loci with CRISPR Interference. Methods Mol Biol. 2020;2161:1-16 pubmed publisher
Stolze L, Conklin A, Whalen M, López Rodríguez M, Ounap K, Selvarajan I, et al. Systems Genetics in Human Endothelial Cells Identifies Non-coding Variants Modifying Enhancers, Expression, and Complex Disease Traits. Am J Hum Genet. 2020;106:748-763 pubmed publisher
Coe E, Tan J, Shapiro M, Louphrasitthiphol P, Bassett A, Marques A, et al. The MITF-SOX10 regulated long non-coding RNA DIRC3 is a melanoma tumour suppressor. PLoS Genet. 2019;15:e1008501 pubmed publisher
Forstnerič V, Oven I, Ogorevc J, Lainšček D, Praznik A, Lebar T, et al. CRISPRa-mediated FOXP3 gene upregulation in mammalian cells. Cell Biosci. 2019;9:93 pubmed publisher
Luo H, Zhu G, Xu J, Lai Q, Yan B, Guo Y, et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice. Cancer Cell. 2019;36:645-659.e8 pubmed publisher
Quan L, Zhang C, Dong M, Jiang J, Xu H, Yan C, et al. Myristoleic acid produced by enterococci reduces obesity through brown adipose tissue activation. Gut. 2020;69:1239-1247 pubmed publisher
Soubeyrand S, Lau P, Peters V, McPherson R. Off-target effects of CRISPRa on interleukin-6 expression. PLoS ONE. 2019;14:e0224113 pubmed publisher
Wang G, Hu H, Chang Y, Huang Y, Song Z, Zhou S, et al. Rab7 regulates primary cilia disassembly through cilia excision. J Cell Biol. 2019;218:4030-4041 pubmed publisher
Chen J, Nathaniel D, Raghavan P, Nelson M, Tian R, Tse E, et al. Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. J Biol Chem. 2019;294:18952-18966 pubmed publisher
Bassaganyas L, Popa S, Horlbeck M, Puri C, Stewart S, Campelo F, et al. New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells. J Cell Biol. 2019;218:3861-3879 pubmed publisher
Tian R, Gachechiladze M, Ludwig C, Laurie M, Hong J, Nathaniel D, et al. CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons. Neuron. 2019;104:239-255.e12 pubmed publisher
Elguindy M, Kopp F, Goodarzi M, Rehfeld F, Thomas A, Chang T, et al. PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. elife. 2019;8: pubmed publisher
Sher F, HOSSAIN M, Seruggia D, Schoonenberg V, Yao Q, Cifani P, et al. Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis. Nat Genet. 2019;51:1149-1159 pubmed publisher
Lainšček D, Kadunc L, Mancek Keber M, Hafner Bratkovic I, Romih R, Jerala R. Delivery of an artificial transcription regulator dCas9-VPR by extracellular vesicles for therapeutic gene activation. ACS Synth Biol. 2018;: pubmed publisher
Zaini M, Patel S, Syafruddin S, Rodrigues P, Vanharanta S. Endogenous HIF2A reporter systems for high-throughput functional screening. Sci Rep. 2018;8:12063 pubmed publisher
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Stojic L, Lun A, Mangei J, Mascalchi P, Quarantotti V, Barr A, et al. Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis. Nucleic Acids Res. 2018;46:5950-5966 pubmed publisher
Shurtleff M, Itzhak D, Hussmann J, Schirle Oakdale N, Costa E, Jonikas M, et al. The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. elife. 2018;7: pubmed publisher
Lin L, Liu Y, Xu F, Huang J, Daugaard T, Petersen T, et al. Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. Gigascience. 2018;7:1-19 pubmed publisher
Hill A, McFaline Figueroa J, Starita L, Gasperini M, Matreyek K, Packer J, et al. On the design of CRISPR-based single-cell molecular screens. Nat Methods. 2018;15:271-274 pubmed publisher
Verma N, Pan H, Dore L, Shukla A, Li Q, Pelham Webb B, et al. TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells. Nat Genet. 2018;50:83-95 pubmed publisher
Mumbach M, Satpathy A, Boyle E, Dai C, Gowen B, Cho S, et al. Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements. Nat Genet. 2017;49:1602-1612 pubmed publisher
Batra R, Nelles D, Pirie E, Blue S, Marina R, Wang H, et al. Elimination of Toxic Microsatellite Repeat Expansion RNA by RNA-Targeting Cas9. Cell. 2017;170:899-912.e10 pubmed publisher
Mou H, Smith J, Peng L, Yin H, Moore J, Zhang X, et al. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017;18:108 pubmed publisher
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, et al. Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. Nat Commun. 2017;8:14725 pubmed publisher
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Limpitikul W, Dick I, Tester D, Boczek N, Limphong P, Yang W, et al. A Precision Medicine Approach to the Rescue of Function on Malignant Calmodulinopathic Long-QT Syndrome. Circ Res. 2017;120:39-48 pubmed publisher
Lebar T, Jerala R. Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits. ACS Synth Biol. 2016;5:1050-1058 pubmed
Evers B, Jastrzebski K, Heijmans J, Grernrum W, Beijersbergen R, Bernards R. CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes. Nat Biotechnol. 2016;34:631-3 pubmed publisher
Shen F, Zhang Y, Jernigan D, Feng X, Yan J, Garcia F, et al. Novel Small-Molecule CX3CR1 Antagonist Impairs Metastatic Seeding and Colonization of Breast Cancer Cells. Mol Cancer Res. 2016;14:518-27 pubmed publisher
Nelles D, Fang M, O Connell M, Xu J, Markmiller S, Doudna J, et al. Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell. 2016;165:488-96 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Gao X, Tsang J, Gaba F, Wu D, Lu L, Liu P. Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Nucleic Acids Res. 2014;42:e155 pubmed publisher
Kabadi A, Gersbach C. Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods. 2014;69:188-97 pubmed publisher
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product information
Catalog Number :
46911
Product Name :
pHR-SFFV-dCas9-BFP-KRAB
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_originAddgene
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
This plasmid was used in the experiments requiring stable expression of KRAB-dCas9 fusion protein from Gilbert et al., 2014 Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159(3):647-61 PubMed ID: 25307932
growth strain :
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3CCAGAGGTTGATTATCGATAAGC
sequencing_primer_5gcttcccgagctctataaaagag
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9-BFP-KRAB fusion
shRNA_sequence
size5172
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
locationC terminal on insert
tag2xNLS
locationC terminal on insert
tagBFP
locationC terminal on insert
tagKRAB domain
plasmid copy :
The Cas9 gene was a gift from Martin Jinek and Jennifer Doudna
resistance markers :
1487
1648
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA