This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHR-SFFV-dCas9-BFP
catalog :
46910
citations: 7
Reference
Mou H, Smith J, Peng L, Yin H, Moore J, Zhang X, et al. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017;18:108 pubmed publisher
Liu Q, Miller L, Blecha F, Sang Y. Reduction of infection by inhibiting mTOR pathway is associated with reversed repression of type I interferon by porcine reproductive and respiratory syndrome virus. J Gen Virol. 2017;98:1316-1328 pubmed publisher
Hung V, Lam S, Udeshi N, Svinkina T, Guzman G, Mootha V, et al. Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. elife. 2017;6: pubmed publisher
Huang Y, Zhou B, Wernig M, Sudhof T. ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and A? Secretion. Cell. 2017;168:427-441.e21 pubmed publisher
Wu R, Nie Q, Tapper E, Jerde C, Dunlap G, Shrestha S, et al. Histone H3K27 Trimethylation Modulates 5-Fluorouracil Resistance by Inhibiting PU.1 Binding to the DPYD Promoter. Cancer Res. 2016;76:6362-6373 pubmed
Kiani S, Beal J, Ebrahimkhani M, Huh J, Hall R, Xie Z, et al. CRISPR transcriptional repression devices and layered circuits in mammalian cells. Nat Methods. 2014;11:723-6 pubmed publisher
Gilbert L, Larson M, Morsut L, Liu Z, Brar G, Torres S, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013;154:442-51 pubmed publisher
product information
Catalog Number :
46910
Product Name :
pHR-SFFV-dCas9-BFP
article :
doi10.1016/j.cell.2013.06.044
id7026
pubmed_id23849981
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_originAddgene
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3CCAGAGGTTGATTATCGATAAGC
sequencing_primer_5gcttcccgagctctataaaagag
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9-BFP fusion
shRNA_sequence
size4935
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
locationC terminal on insert
tag2xNLS
locationC terminal on insert
tagBFP
plasmid copy :
The Cas9 gene was a gift from Martin Jinek and Jennifer Doudna
resistance markers :
1487
1648
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA