pRK5-EGFP-Tau E14 P301L | Addgene 46909 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5-EGFP-Tau E14 P301L

catalog :

46909

citations: 1

Reference
Hoover B, Reed M, Su J, Penrod R, Kotilinek L, Grant M, et al. Tau mislocalization to dendritic spines mediates synaptic dysfunction independently of neurodegeneration. Neuron. 2010;68:1067-81 pubmed publisher

product information

Catalog Number :

46909

Product Name :

pRK5-EGFP-Tau E14 P301L

article :

doi	10.1016/j.neuron.2010.11.030
id	6986
pubmed_id	21172610

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

WT htau construct (Addgene plasmid 46904) was used as a template to make mutations and contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9 13, intron 13, and exon 14.

growth strain :

expresses EGFP tagged Tau E14 P301L in mammalian cells

origin :

pi :

alt_names

Tau

microtubule-associated protein tau

MAPT

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	Cla1
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
gene	MAPT
id	4137

genbank_ids

mutation

P301L AND all 14 S/P or T/P amino acid residues (T111, T153, T175, T181, S199, S202, T205, T212, T217, T231, S235, S396, S404, and S422; numbering based on the longest 441-amino acid brain isoform of htau) changed to glutamate

name

Tau E14 P301L

shRNA_sequence

size

species

9606

Homo sapiens