pRK5-EGFP-Tau E14 | Addgene 46907 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5-EGFP-Tau E14

catalog :

46907

citations: 7

Reference
Lopez D, Maltby C, Warming H, Divecha N, Vargas Caballero M, Coldwell M, et al. A luminescence-based reporter to study tau secretion reveals overlapping mechanisms for the release of healthy and pathological tau. Front Neurosci. 2023;17:1196007 pubmed publisher
Peinado J, Chaplot K, Jarvela T, Barbieri E, Shorter J, Lindberg I. Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. ACS Chem Neurosci. 2022;13:1651-1665 pubmed publisher
Hallinan G, Vargas Caballero M, West J, Deinhardt K. Tau Misfolding Efficiently Propagates between Individual Intact Hippocampal Neurons. J Neurosci. 2019;39:9623-9632 pubmed publisher
Vaz Silva J, Gomes P, Jin Q, Zhu M, Zhuravleva V, Quintremil S, et al. Endolysosomal degradation of Tau and its role in glucocorticoid-driven hippocampal malfunction. EMBO J. 2018;37: pubmed publisher
Rodriguez Rodriguez P, Sandebring Matton A, Merino Serrais P, Parrado Fernandez C, Rabano A, Winblad B, et al. Tau hyperphosphorylation induces oligomeric insulin accumulation and insulin resistance in neurons. Brain. 2017;: pubmed publisher
Hatch R, Wei Y, Xia D, Götz J. Hyperphosphorylated tau causes reduced hippocampal CA1 excitability by relocating the axon initial segment. Acta Neuropathol. 2017;133:717-730 pubmed publisher
Hoover B, Reed M, Su J, Penrod R, Kotilinek L, Grant M, et al. Tau mislocalization to dendritic spines mediates synaptic dysfunction independently of neurodegeneration. Neuron. 2010;68:1067-81 pubmed publisher

product information

Catalog Number :

46907

Product Name :

pRK5-EGFP-Tau E14

article :

doi	10.1016/j.neuron.2010.11.030
id	6986
pubmed_id	21172610

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

WT htau construct (Addgene plasmid 46904) was used as a template to make mutations and contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9 13, intron 13, and exon 14.

growth strain :

expresses EGFP tagged Tau E14 in mammalian cells

origin :

pi :

alt_names

Tau

microtubule-associated protein tau

MAPT

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	Cla1
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
gene	MAPT
id	4137

genbank_ids

mutation

all 14 S/P or T/P amino acid residues (T111, T153, T175, T181, S199, S202, T205, T212, T217, T231, S235, S396, S404, and S422; numbering based on the longest 441-amino acid brain isoform of htau) to glutamate

name

Tau E14

shRNA_sequence

size

species

9606

Homo sapiens