This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5-EGFP-Tau
catalog :
46904
citations: 31
Reference
Lopez D, Maltby C, Warming H, Divecha N, Vargas Caballero M, Coldwell M, et al. A luminescence-based reporter to study tau secretion reveals overlapping mechanisms for the release of healthy and pathological tau. Front Neurosci. 2023;17:1196007 pubmed publisher
Minckley T, Salvagio L, Fudge D, VERHEY K, Markus S, Qin Y. Zn2+ decoration of microtubules arrests axonal transport and displaces tau, doublecortin, and MAP2C. J Cell Biol. 2023;222: pubmed publisher
Tao X, Liu J, Diaz Perez Z, Foley J, Stewart T, Casero R, et al. Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in Tauopathy. bioRxiv. 2023;: pubmed publisher
Bhagat R, Minaya M, Renganathan A, Mehra M, Marsh J, Martinez R, et al. Long non-coding RNA SNHG8 drives stress granule formation in tauopathies. medRxiv. 2023;: pubmed publisher
Rajbanshi B, Guruacharya A, Mandell J, Bloom G. Localization, induction, and cellular effects of tau phosphorylated at threonine 217. Alzheimers Dement. 2023;19:2874-2887 pubmed publisher
Hill S, Esquivel A, Ospina S, Rahal L, Dickey C, Blair L. Chaperoning activity of the cyclophilin family prevents tau aggregation. Protein Sci. 2022;31:e4448 pubmed publisher
Kim K, Park S, Hong J, Koh J, Cho D, Hwang J, et al. Zinc enhances autophagic flux and lysosomal function through transcription factor EB activation and V-ATPase assembly. Front Cell Neurosci. 2022;16:895750 pubmed publisher
Peinado J, Chaplot K, Jarvela T, Barbieri E, Shorter J, Lindberg I. Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. ACS Chem Neurosci. 2022;13:1651-1665 pubmed publisher
Norambuena A, Sun X, Wallrabe H, Cao R, Sun N, Pardo E, et al. SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers. Neurobiol Dis. 2022;169:105737 pubmed publisher
Torres Garcia L, P Domingues J, Brandi E, Haikal C, Mudannayake J, Brás I, et al. Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation. Sci Rep. 2022;12:2987 pubmed publisher
Cook B, Walker N, Zhang Q, Chen S, Evans T. The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3-beta signaling. J Biol Chem. 2021;296:100696 pubmed publisher
Paul S, Dansithong W, Figueroa K, Gandelman M, Scoles D, Pulst S. Staufen1 in Human Neurodegeneration. Ann Neurol. 2021;89:1114-1128 pubmed publisher
Giovinazzo D, Bursać B, Sbodio J, Nalluru S, Vignane T, Snowman A, et al. Hydrogen sulfide is neuroprotective in Alzheimer's disease by sulfhydrating GSK3β and inhibiting Tau hyperphosphorylation. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Mahendran T, Suresh S, Garimella L, Manjithaya R. Soluble 4R0N Tau Abrogates Endocytic Vesicular Dynamics. Front Aging Neurosci. 2020;12:537712 pubmed publisher
Park L, Hochrainer K, Hattori Y, Ahn S, Anfray A, Wang G, et al. Tau induces PSD95-neuronal NOS uncoupling and neurovascular dysfunction independent of neurodegeneration. Nat Neurosci. 2020;23:1079-1089 pubmed publisher
Sonntag T, Moresco J, Yates J, Montminy M. The KLDpT activation loop motif is critical for MARK kinase activity. PLoS ONE. 2019;14:e0225727 pubmed publisher
Hallinan G, Vargas Caballero M, West J, Deinhardt K. Tau Misfolding Efficiently Propagates between Individual Intact Hippocampal Neurons. J Neurosci. 2019;39:9623-9632 pubmed publisher
Li D, Cho Y. High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay. J Neurochem. 2020;152:122-135 pubmed publisher
Teravskis P, Oxnard B, Miller E, Kemper L, Ashe K, Liao D. Phosphorylation in two discrete tau domains regulates a stepwise process leading to postsynaptic dysfunction. J Physiol. 2019;: pubmed publisher
Makhoul C, Gosavi P, Duffield R, Delbridge B, Williamson N, Gleeson P. Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture. Mol Biol Cell. 2019;30:370-386 pubmed publisher
Vaz Silva J, Gomes P, Jin Q, Zhu M, Zhuravleva V, Quintremil S, et al. Endolysosomal degradation of Tau and its role in glucocorticoid-driven hippocampal malfunction. EMBO J. 2018;37: pubmed publisher
St Amand M, Bond M, Riedy J, Comly M, Shiloach J, Hanover J. A genetic model to study O-GlcNAc cycling in immortalized mouse embryonic fibroblasts. J Biol Chem. 2018;293:13673-13681 pubmed publisher
Li D, Wang L, Maziuk B, Yao X, Wolozin B, Cho Y. Directed evolution of a picomolar-affinity, high-specificity antibody targeting phosphorylated tau. J Biol Chem. 2018;293:12081-12094 pubmed publisher
Xu Y, Cui L, DiBello A, Wang L, Lee J, Saidi L, et al. DNAJC5 facilitates USP19-dependent unconventional secretion of misfolded cytosolic proteins. Cell Discov. 2018;4:11 pubmed publisher
Lu M, Liu T, Jiao Q, Ji J, Tao M, Liu Y, et al. Discovery of a Keap1-dependent peptide PROTAC to knockdown Tau by ubiquitination-proteasome degradation pathway. Eur J Med Chem. 2018;146:251-259 pubmed publisher
Gosavi P, Houghton F, McMillan P, Hanssen E, Gleeson P. The Golgi ribbon in mammalian cells negatively regulates autophagy by modulating mTOR activity. J Cell Sci. 2018;131: pubmed publisher
Rodriguez Rodriguez P, Sandebring Matton A, Merino Serrais P, Parrado Fernandez C, Rabano A, Winblad B, et al. Tau hyperphosphorylation induces oligomeric insulin accumulation and insulin resistance in neurons. Brain. 2017;: pubmed publisher
Wang Y, Hall R, Lee M, Kamgar Parsi A, Bi X, Baudry M. The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation. Sci Rep. 2017;7:11771 pubmed publisher
Ji C, Tang M, Johnson G. Assessing the degradation of tau in primary neurons: The role of autophagy. Methods Cell Biol. 2017;141:229-244 pubmed publisher
Lasagna Reeves C, de Haro M, Hao S, Park J, Rousseaux M, Al Ramahi I, et al. Reduction of Nuak1 Decreases Tau and Reverses Phenotypes in a Tauopathy Mouse Model. Neuron. 2016;92:407-418 pubmed publisher
Hoover B, Reed M, Su J, Penrod R, Kotilinek L, Grant M, et al. Tau mislocalization to dendritic spines mediates synaptic dysfunction independently of neurodegeneration. Neuron. 2010;68:1067-81 pubmed publisher
product information
Catalog Number :
46904
Product Name :
pRK5-EGFP-Tau
article :
doi10.1016/j.neuron.2010.11.030
id6986
pubmed_id21172610
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses EGFP tagged Tau in mammalian cells
origin :
37
pi :
alt_names
Tau
microtubule-associated protein tau
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5Cla1
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
geneMAPT
id4137
genbank_ids
mutationThis WT htau construct contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9 13, intron 13, and exon 14
nameMAPT
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
1642
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA