This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
psd44-GqQL
catalog :
46826
citations: 3
Reference
Cabezudo S, Sanz Flores M, Caballero A, Tasset I, Rebollo E, Díaz A, et al. Gαq activation modulates autophagy by promoting mTORC1 signaling. Nat Commun. 2021;12:4540 pubmed publisher
Lopez Mosqueda J, Maddi K, Prgomet S, Kalayil S, Marinovic Terzic I, Terzic J, et al. SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks. elife. 2016;5: pubmed publisher
Hashimoto S, Mikami S, Sugino H, Yoshikawa A, Hashimoto A, Onodera Y, et al. Lysophosphatidic acid activates Arf6 to promote the mesenchymal malignancy of renal cancer. Nat Commun. 2016;7:10656 pubmed publisher
product information
Catalog Number :
46826
Product Name :
psd44-GqQL
article :
doi
id6965
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepsd44
backbone_mutationpRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
backbone_originDr. Richard Iggo, University of St Andrews, Edinburgh, UK
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
cDNA to make this plasmid was obtained from www.cdna.org (ID GNA0Q000C0). Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.
growth strain :
2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
origin :
37
pi :
alt_names
GNAQ
guanine nucleotide binding protein (G protein), q polypeptide
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5BamHI
promoterPGK promoter
sequencing_primer_3
sequencing_primer_5hPGK-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMAL, CMC1, G-ALPHA-q, GAQ, SWS
geneGNAQ
id2776
genbank_ids
mutationQ209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
nameg protein alpha q
shRNA_sequence
size1120
species
9606
Homo sapiens
tags
resistance markers :
1638
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA