This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFA3
catalog :
46790
citations: 1
product information
Catalog Number :
46790
Product Name :
pFA3
article :
| doi | 10.1371/journal.pone.0008111 |
| id | 6956 |
| pubmed_id | 19956593 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pYES2.1/V5-His/lacZ | |
| backbone_mutation | The vector used for galactose-induced yeast expression of C-terminal double tagged (V5 and 6xHis) proteins was the pYES2.1/V5-His-TOPO (Invitrogen) and cloning was according to the manufacturer s specifications. The identity of the cloned material was confirmed by restriction digestion analysis and DNA sequencing. PFA3 contains the ATPase-inactive version of Fun30, which carries a point mutation in the ATPase domain (Corona et al, 1999) that replaces lysine at position 603 (AAA) by arginine (AGA). The point mutation was introduced with the kit QuickChange XL Site-Directed Mutagenesis (Stratagene), according to the manufacturer s specifications. The template was PFA1 and the primers were Mut forward (CGACATGGGTCTAGGTAGAACATGTCAAGTCATTTC) and Mut reverse (GAAATGACTTGACATGTTCTACCTAGACCCATGTCG). | |
| backbone_origin | Invitrogen | |
| backbone_size | 8964 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
To express ATPase inactive Fun30 in budding yeast
origin :
37
pi :
|
resistance markers :
| 1634 |
tags :
Unknown
terms :
| URA3 |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments