PCMV-intron myc Rab11 S25N | Addgene 46786 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PCMV-intron myc Rab11 S25N

catalog :

46786

citations: 2

Reference
Melendez J, Sieiro D, Salgado D, Morin V, Dejardin M, Zhou C, et al. TGFβ signalling acts as a molecular brake of myoblast fusion. Nat Commun. 2021;12:749 pubmed publisher
Dupre D, Robitaille M, Ethier N, Villeneuve L, Mamarbachi A, Hebert T. Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking. J Biol Chem. 2006;281:34561-73 pubmed

product information

Catalog Number :

46786

Product Name :

PCMV-intron myc Rab11 S25N

article :

doi	10.1074/jbc.M605012200
id	6954
pubmed_id	16959776

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-intron myc

backbone_mutation

intron-myc inserted HindIII/XbaI. Intron enhances stability of mRNA.

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

) BGH RVS primer (5 -TAGAAGGCACAGTCGAGG-3 )
ACGACTACCTCTTTAAAGTTGTCCTTATTGGAGATTCTG
GTGTTGGAAAGAATAATCTCCTGTCT-3
cDNA was obtained from UMR cDNA Resource Center, www.cdna.org To make mutation, the following primers were used for PCR amplification: Rab11 S25N FWD (5 -AGCTCGGATCCACCATGGGCACCCGCGACGACGAGT

growth strain :

expression of S25N Rab11a in mammalian cells

origin :

pi :

alt_names

YL8

RAB11A, member RAS oncogene family

RAB11A

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGH Reverse
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YL8
gene	RAB11A
id	8766

genbank_ids

mutation

S25N dominant negative

name

RAB11A S25N

shRNA_sequence

size

650

species

9606

Homo sapiens