This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PCMV-intron myc Rab6 T27N
catalog :
46782
citations: 3
Reference
Frisbie C, Lushnikov A, Krasnoslobodtsev A, Riethoven J, Clarke J, Stepchenkova E, et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells. 2019;8: pubmed publisher
Petrosyan A, Casey C, Cheng P. The role of Rab6a and phosphorylation of non-muscle myosin IIA tailpiece in alcohol-induced Golgi disorganization. Sci Rep. 2016;6:31962 pubmed publisher
Dupre D, Robitaille M, Ethier N, Villeneuve L, Mamarbachi A, Hebert T. Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking. J Biol Chem. 2006;281:34561-73 pubmed
product information
Catalog Number :
46782
Product Name :
PCMV-intron myc Rab6 T27N
article :
doi10.1074/jbc.M605012200
id6954
pubmed_id16959776
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-intron myc
backbone_mutationintron-myc inserted HindIII/XbaI. Intron enhances stability of mRNA.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
) or Rab6 T27N RVS (5 -TCTGGTGATCAAAGAATTCTTTCCAACGCTTTGCTCCCCCA-3 ).
-AGCAAAGCGTTGGAAAGAATTGTTTGATCATCAGATTC
ATG-3
cDNA was obtained from UMR cDNA Resource Center, www.cdna.org Rab6a T27N was created by a series of PCRs using T7, BGH RVS primers as well as the Rab6T27N-specific primers as follows: FWD (5
growth strain :
expression of T27N Rab6a in mammalian cells
origin :
37
pi :
alt_names
Rab6A
member RAS oncogene family
Rab6
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRAB6
geneRAB6A
id5870
genbank_ids
mutationT27N dominant negative
nameRab6 T27N
shRNA_sequence
size640
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyc
resistance markers :
1635
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA