This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PCMV-intron myc Rab2 S20N
catalog :
46780
citations: 1
Reference
Dupre D, Robitaille M, Ethier N, Villeneuve L, Mamarbachi A, Hebert T. Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking. J Biol Chem. 2006;281:34561-73 pubmed
product information
Catalog Number :
46780
Product Name :
PCMV-intron myc Rab2 S20N
article :
doi10.1074/jbc.M605012200
id6954
pubmed_id16959776
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-intron myc
backbone_mutationintron-myc inserted HindIII/XbaI. Intron enhances stability of mRNA.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
), BGH RVS primer (5 -TAGAAGGCACAGTCGAGG-3 )
-AGCTCGGATCCACCATGGCGTACGCCTATCTCTTCAAG
TACATCATAATCGGCGACACAGGTGTTGGTAAAAACTGC
TTATTGCT-3
cDNA was obtained from UMR cDNA Resource Center, www.cdna.org To make mutation, the following primers were used for PCR amplification: Rab2 S20N FWD (5
growth strain :
expression of S20N Rab2a in mammalian cells
origin :
37
pi :
alt_names
RAB2A
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLHX, RAB2
geneRAB2A
id5862
genbank_ids
mutationS20N dominant negative
nameRAB2
shRNA_sequence
size640
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyc
resistance markers :
1635
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA