PCMV-intron myc Rab1a S25N | Addgene 46777 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PCMV-intron myc Rab1a S25N

catalog :

46777

citations: 2

Reference
Webster C, Smith E, Bauer C, Moller A, Hautbergue G, Ferraiuolo L, et al. The C9orf72 protein interacts with Rab1a and the ULK1 complex to regulate initiation of autophagy. EMBO J. 2016;35:1656-76 pubmed publisher
Dupre D, Robitaille M, Ethier N, Villeneuve L, Mamarbachi A, Hebert T. Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking. J Biol Chem. 2006;281:34561-73 pubmed

product information

Catalog Number :

46777

Product Name :

PCMV-intron myc Rab1a S25N

article :

doi	10.1074/jbc.M605012200
id	6954
pubmed_id	16959776

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-intron myc

backbone_mutation

intron-myc inserted HindIII/XbaI. Intron enhances stability of mRNA.

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

) BGH RVS primer (5 -TAGAAGGCACAGTCGAGG-3 )
-AGCTCGGATCCACCATGTCCAGCATGAATCCCGAATAT
GATTATTTATTCAAGTTACTTCTGATTGGCGACTCAGGG
GTTGGAAAGAATTGCCTTCTTCT-3
cDNA was obtained from UMR cDNA Resource Center, www.cdna.org To make mutation, the following primers were used for PCR amplification: Rab1 S25N FWD (5

growth strain :

expression of S25N Rab1a in mammalian cells

origin :

pi :

alt_names

member RAS oncogene family

Rab1a

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGH Reverse
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RAB1, YPT1
gene	RAB1A
id	5861

genbank_ids

mutation

S25N (dominant negative)

name

Rab1a S25N mutant

shRNA_sequence

size

species

9606

Homo sapiens