This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLIGFP_W220F
catalog :
46772
citations: 1
Reference
Gatti Lafranconi P, Dijkman W, Devenish S, Hollfelder F. A single mutation in the core domain of the lac repressor reduces leakiness. Microb Cell Fact. 2013;12:67 pubmed publisher
product information
Catalog Number :
46772
Product Name :
pLIGFP_W220F
article :
doi10.1186/1475-2859-12-67
id6921
pubmed_id23834731
bacterial resistance :
Ampicillin
cloning :
backbonepET22b
backbone_mutationPosition 220 in LacI is mutated from tryptophan to phenylalanine
backbone_originNovagen
backbone_size5493
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Use BL21 for expression. Growth temperature depends on the protein being over-expressed and is not affected by the modified repressor LacIWF.
growth strain :
Protein overexpression with the modified LacIWF repressor
origin :
37
pi :
alt_names
LacIWF
LacI
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3aggctctcaagggcatcgg
sequencing_primer_5cgccattcgatggtgtcc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
mutationTryptophan 220 replaced by Phenylalanine
nameLacI_W220F
shRNA_sequence
size1083
species
tags
resistance markers :
1639
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA