This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNAI-GAL4-CREB-S133A
catalog :
46770
citations: 2
Reference
Barbieri I, Tzelepis K, Pandolfini L, Shi J, Millán Zambrano G, Robson S, et al. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature. 2017;552:126-131 pubmed publisher
Sun P, Enslen H, Myung P, Maurer R. Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity. Genes Dev. 1994;8:2527-39 pubmed
product information
Catalog Number :
46770
Product Name :
pcDNAI-GAL4-CREB-S133A
article :
doi10.1101/gad.8.21.2527
id6616
pubmed_id7958915
bacterial resistance :
Ampicillin
cloning :
backbonepcDNAI-AMP
backbone_mutation
backbone_originInvitrogen
backbone_size4767
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of GAL4 fusion to CREB-S133A
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3sp6
sequencing_primer_5t7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCreb
geneCreb1
id81646
aliasesYPL248C, GAL81
geneGAL4
id855828
genbank_ids
NM_001184062.1
NM_134443.1
mutationChanged CREB serine 133 to Alanine
nameGAL4(1-147)-CREB-S133A
shRNA_sequence
size1500
species
10116
Rattus norvegicus
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagGAL4
plasmid copy :
The rat CREB coding sequence was obtained by PCR from mRNA. The GAL4(1-147) coding sequence was obtained from PG424 (Sadowski & Ptashne, Nucleic Acids Res. 17:7539, 1989) which was provided by Dr. Ptashne.
resistance markers :
1631
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA